Inhibition of Low-Density Lipoprotein Oxidation by Nagano Purple Grape (Vitis vinifera x Vitis labrusca)
Inhibition of Low-Density Lipoprotein Oxidation by Nagano Purple Grape (Vitis vinifera x Vitis labrusca)
复制标题
DOI：
10.3177/jnsv.55.471
复制
发表时间：
2009-12-01
影响因子：
1.6
通讯作者：
Kondo, Kazuo
中科院分区：
文献类型：
--
作者：
Kamiyama, Masumi;Kishimoto, Yoshimi;Kondo, Kazuo
长野紫葡萄（葡萄属（V）viniferaXV labrusca）是由巨峰（V viniferaXV labrusca）和罗萨里奥比安科（V vinifera）葡萄杂交而成的杂交品种。葡萄，包括它的皮，都可以吃。并且由于赤霉素处理而不含种子。各种水果的皮已被证明含有抗氧化活性，但目前还不清楚长野紫葡萄是否含有抗氧化活性。我们制备长野紫葡萄的皮和干果（包括皮），以测定抗氧化活性的存在。我们检测了葡萄中多酚的浓度，并进一步分析了葡萄中的成分是否抑制了低密度脂蛋白（LDL）的氧化。我们检测到花青素-3-葡萄糖苷（Cy-3-glc），五种花青素和白藜芦醇的存在。在果肉中检测到微量白藜芦醇。从人类受试者1小时后的消费的皮肤或干果收集的LDL揭示了显着的抑制LDL氧化相比，在空腹静脉血样品中观察到的。进一步观察了Cy-3-glc的抗氧化活性。我们的研究结果表明，长野紫葡萄的消费可以产生抗LDL氧化。
The Nagano Purple grape (Vitis (V) viniferaXV labrusca) is a hybrid created by a cross between Kyoho (V viniferaXV labrusca) and Rosario Bianco (V vinifera) grapes. The grape, including its skin, can be eaten. and contains no seeds because of gibberellin treatment. The skins of various fruits have been shown to contain antioxidant activity However, it is unclear whether the Nagano Purple grape contains antioxidant activity. We prepared the skins and dried fruits (including the skins) of the Nagano Purple grape, so as to assay for the presence of an antioxidant activity. We examined the concentration of polyphenols in the grape and further assayed whether components in the grape inhibited the oxidation of low-density lipoprotein (LDL). We detected the presence of cyanidin-3-glucoside (Cy-3-glc), five anthocyanidins and resveratrol in the skins. A trace of resveratrol was detected in the pulp. LDL collected from human Subjects 1 h following the consumption of the skins or dried fruits revealed significant inhibition of LDL oxidation compared to that observed in fasting venous blood samples. We further observed the antioxidant activity of Cy-3-glc. Our results suggest that the consumption of the Nagano Purple grape can give rise to resistance to LDL oxidation.