Elemene injection induced autophagy protects human hepatoma cancer cells from starvation and undergoing apoptosis.
Elemene injection induced autophagy protects human hepatoma cancer cells from starvation and undergoing apoptosis.
复制标题
榄香烯注射液诱导自噬保护人肝癌细胞免于饥饿和凋亡
DOI：
10.1155/2014/637528
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Cai X
中科院分区：
文献类型：
--
作者：
Lin Y;Wang K;Hu C;Lin L;Qin S;Cai X
榄香烯是一种在中药中发现的化合物，对广谱肿瘤具有良好的抗癌作用。在体内实验中，我们发现阿帕替尼（一种选择性抑制VEGFR2的酪氨酸激酶抑制剂）与榄香烯注射液（Ele）联合治疗小鼠H22实体瘤的效果比阿帕替尼单独治疗差。Ele对无血清饥饿诱导的HepG2细胞死亡具有保护作用。进一步的机制研究表明，Ele诱导保护性自噬，阻止人肝癌细胞凋亡。Ele的促凋亡作用在羟氯喹抑制其促自噬作用时增强。总之，Ele具有保护癌细胞免于在阿帕替尼诱导的营养缺乏环境或无血清诱导的饥饿中死亡的作用。因此，榄香烯注射液与自噬抑制剂的组合可能是肝细胞癌的一个有用的治疗选择。
Elemene, a compound found in an herb used in traditional Chinese medicine, has shown promising anticancer effects against a broad spectrum of tumors. In an in vivo experiment, we found that apatinib, a tyrosine kinase inhibitor that selectively inhibits VEGFR2, combined with elemene injection (Ele) for the treatment of H22 solid tumor in mice resulted in worse effectiveness than apatinib alone. Moreover, Ele could protect HepG2 cells from death induced by serum-free starvation. Further data on the mechanism study revealed that Ele induced protective autophagy and prevented human hepatoma cancer cells from undergoing apoptosis. Proapoptosis effect of Ele was enhanced when proautophagy effect was inhibited by hydroxychloroquine. Above all, Ele has the effect of protecting cancer cells from death either in apatinib induced nutrient deficient environment or in serum-free induced starvation. A combination of elemene injection with autophagy inhibitor might thus be a useful therapeutic option for hepatocellular carcinoma.