Tubular basement membrane changes in 2-amino-4,5-diphenylthiazole-induced polycystic disease.
Tubular basement membrane changes in 2-amino-4,5-diphenylthiazole-induced polycystic disease.
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2-氨基-4,5-二苯基噻唑诱导的多囊病中管状基底膜的变化。
DOI：
10.1038/ki.1985.193
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发表时间：
1985
影响因子：
19.6
通讯作者：
Hudson,BG
中科院分区：
文献类型：
--
作者：
Butkowski,RJ;Carone,FA;Grantham,JJ;Hudson,BG
2-氨基-4,5-二苯基噻唑诱导多囊性疾病的管状基底膜改变。用2-氨基-4,5-二苯噻唑诱导大鼠多囊性肾病。对肾小管（TBM）和肾小球基底膜（GBM）进行纯化并分析其可能的结构变化，这些变化可能是肾小管扩张和囊肿发展的一个因素。采用十二烷基硫酸钠聚丙烯酰胺凝胶电泳法检测TBM多肽相对量的变化。观察到高分子量组分的浓度总体上升，低分子量组分的浓度下降。特别值得注意的变化包括Mr= 380,000的成分增加了两倍，Mr= 55,000的成分减少了一倍，但没有减少二硫键。随着二硫键的减少，Mr= 380,000的组分解离，而Mr= 55,000的多肽则不解离，Mr= 245,000和145,000的多肽的浓度增加了约两倍（近似分子量是用球状蛋白标准测定的）。这些变化在给药4至8周期间发生得最为迅速，在8至16周期间保持相对稳定。如果停止给药，TBM多肽的分布恢复正常。以上结果提示，2-氨基-4,5-二苯基噻唑致多囊肾病动物的管状基底膜异常，这可能是导致囊肿形成的一个因素。
Tubular basement membrane changes in 2-amino-4,5-diphenylthiazole-induced polycystic disease. Polycystic renal disease was induced in rats by feeding 2-amino-4,5-diphenylthiazole. Tubular (TBM) and glomerular basement membranes (GBM) were purified and analyzed for possible structural changes that may be a factor in the development of the tubular dilations and cysts. Changes in the relative quantities of TBM polypeptides were detected by sodium dodecylsulfate polyacrylamide gel electrophoresis. An overall increase in the concentration of high molecular weight components and a decrease in concentration of those of low molecular weight components were observed. Changes which were particularly notable included a twofold increase in a component of Mr= 380,000 and a decrease in one of Mr= 55,000 as analyzed without reduction of disulfide bonds. With reduction of disulfide bonds, the Mr= 380,000 component dissociates, whereas the Mr= 55,000 polypeptide does not, and polypeptides of Mr= 245,000 and 145,000 are observed to increase about twofold in concentration (approximate molecular weights were determined using globular protein standards). These changes take place most rapidly from 4 to 8 weeks of drug administration and remain relatively constant between 8 and 16 weeks. If feeding of the drug is discontinued, the distribution of TBM polypeptides returns to normal. These results indicate that tubular basement membrane from animals with 2-amino-4,5-diphenylthiazole-induced polycystic renal disease is abnormal, and this should be considered as a possible contributing factor in the formation of cysts.