Method for rapid identification of oral streptococci by PCR using 16S-23S ribosomal RNA intergenic spacer gene
Method for rapid identification of oral streptococci by PCR using 16S-23S ribosomal RNA intergenic spacer gene
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DOI：
10.1016/s0917-2394(05)70051-3
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发表时间：
2005-01-01
影响因子：
0.8
通讯作者：
Fujiwara, Taku
中科院分区：
文献类型：
--
作者：
Hoshino, Tomonori;Izumi, Tomohiro;Fujiwara, Taku
变形链球菌群在龋病的形成中起重要作用，而缓症链球菌群被认为与亚急性细菌性心内膜炎有关。缓症链球菌和唾液链球菌是人类口腔中的早期定植者，它们都对变异链球菌的感染和定植产生影响。本研究利用16 S-23 S核糖体RNA基因间隔区基因建立了一种快速、准确的口腔链球菌PCR检测方法。PCR分析能够正确鉴定变形链球菌和缓症链球菌。我们的研究结果表明，在本研究中建立的组合PCR分析方法是有用的，用于检测口腔链球菌物种，以及在临床应用，以预测和预防龋齿和链球菌感染性心内膜炎的病因学研究。
Mutans group streptococci play an important role in the formation of dental caries, while mitis group streptococci are considered to be associated with subacute bacterial endocarditis. Both mitis and salivarius group streptococci, which are early colonizers in the human oral cavity, have an effect on the infection and colonization of mutans streptococci. In the present study, a rapid and exact PCR method to detect those oral streptococci was developed using the 16S-23S ribosomal RNA intergenic spacer gene, which is present between 16S rRNA and 23S rRNA within the RNA operon. The PCR analysis was able to correctly identify mutans and mitis group streptococcal species. Our results indicate that the combined PCR analysis method established in the present study is useful for detecting oral streptococcal species, as well as in clinical applications to predict and prevent dental caries and etiological studies of streptococcal infective endocarditis.