Role of Akt isoforms in HGF-induced invasive growth of human salivary gland cancer cells
Role of Akt isoforms in HGF-induced invasive growth of human salivary gland cancer cells
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DOI：
10.1016/j.bbrc.2008.03.042
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发表时间：
2008-05-23
影响因子：
3.1
通讯作者：
Hamakawa, Hiroyuki
中科院分区：
文献类型：
--
作者：
Hara, Shingo;Nakashiro, Koh-ichi;Hamakawa, Hiroyuki
肝细胞生长因子（HGF）和c-Met的表达与许多人类恶性肿瘤的进展相关。最近的一项研究表明，HGF和c-Met在人涎腺癌组织中表达。在此，我们研究了HGF/c-Met系统在两种人涎腺癌细胞系：绿色荧光蛋白-腺样囊性癌2（GFP-ACC 2）和GFP-ACCM的侵袭性生长中的作用。HGF通过激活PI 3 K/Akt信号通路促进两种细胞系的侵袭性生长。通过Western印迹分析在两种细胞类型中检测到所有Akt同种型（Akt 1、Akt 2和Akt 3）。使用同种型特异性合成的小干扰RNA敲低任何Akt同种型在很大程度上消除了HGF诱导的侵袭性生长。我们的研究结果表明，所有Akt亚型都是HGF刺激的人唾液腺癌细胞侵袭性生长所必需的，并且靶向单一Akt亚型可能有效治疗唾液腺癌。(c)2008年爱思唯尔公司All rights reserved.
The expression of hepatocyte growth factor (HGF) and c-Met is associated with tumor progression in many human malignancies. A recent study demonstrated HGF and c-Met expression in human salivary gland cancer tissues. Here, we investigated the role of the HGF/c-Met system in the invasive growth of two human salivary gland cancer cell lines: green fluorescent protein-adenoid cystic carcinoma 2 (GFP-ACC2) and GFP-ACCM. HGF enhanced the invasive growth of the two cell lines by activating PI3K/Akt signaling. All Akt isoforms (Akt1, Akt2, and Akt3) were detected in both cell types by Western blot analysis. Knockdown of any of the Akt isoforms using isoform-specific synthetic small-interfering RNAs largely abrogated the invasive growth induced by HGF. Our findings suggest that all of the Akt isoforms are required for the HGF-stimulated invasive growth of human salivary gland cancer cells, and that targeting a single Akt isoform could be effective in treating salivary gland cancers. (c) 2008 Elsevier Inc. All rights reserved.