C-13 nuclear magnetic resonance analysis of glucose and citrate end products in an ldhL-ldhD double-knockout strain of Lactobacillus plantarum
C-13 nuclear magnetic resonance analysis of glucose and citrate end products in an ldhL-ldhD double-knockout strain of Lactobacillus plantarum
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DOI：
10.1128/jb.178.24.7311-7315.1996
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发表时间：
1996-12-01
影响因子：
3.2
通讯作者：
Delcour, J
中科院分区：
文献类型：
--
作者：
Ferain, T;Schanck, AN;Delcour, J
我们用C-13核磁共振检测了在植物乳杆菌中敲除这两个LDH基因的代谢后果。与其野生型等基因前体在所有测试条件下都能产生乳酸作为主要代谢物不同，LDH缺失菌株TF103主要产生乙酸乙酯，还发现了包括有机酸(醋酸酯、琥珀酸、丙酮酸和乳酸)、乙醇、2，3-丁二醇和甘露醇在内的多种次生最终产物。
We have examined the metabolic consequences of knocking out the two ldh genes in Lactobacillus plantarum using C-13 nuclear magnetic resonance. Unlike its wild-type isogenic progenitor, which produced lactate as the major metabolite under all conditions tested, ldh null strain TF103 mainly produced acetoin, A variety of secondary end products were also found, including organic acids (acetate, succinate, pyruvate, and lactate), ethanol, 2,3-butanediol, and mannitol.