Manipulation of SlMXl for enhanced carotenoids accumulation and drought resistance in tomato
Manipulation of SlMXl for enhanced carotenoids accumulation and drought resistance in tomato
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DOI：
10.1007/s11434-016-1108-9
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发表时间：
2016-09-01
期刊：
影响因子：
18.9
通讯作者：
Luo, Jie
中科院分区：
文献类型：
--
作者：
Ewas, Mohamed;Gao, Yanqiang;Luo, Jie
植物是人类饮食中营养的终极来源。为了确保为不断增长的世界人口提供足够的高质量食物，需要在作物中单独或组合选择包括提高对压力的耐受性和营养水平在内的特性。在这里，我们报道了编码番茄中一种混合型MYB转录因子的SLMX1，该转录因子通过调节相应途径的关键结构和调控基因来同时调节抗旱性和代谢过程。SLMX1的过表达显著提高了果实的抗旱性，改善了果实的品质，而敲除SLMX1则导致了相反的表型。本研究为利用单个调控基因进行多个有益性状的基因工程提供了一条有效途径，为作物育种提供了一种新的途径。
Plants are the ultimate source of nutrients in the human diet. To ensure adequate availability of high quality food for an increasing world population, traits including improved tolerance of stresses and nutrient levels need to be selected in crops, both individually and in combination. Here we report the identification of SlMX1 encoding a MIXTA-like MYB transcription factor in tomato that simultaneously modulates drought resistance and metabolic processes through regulating key structural and regulatory genes of the corresponding pathways. Over-expression of SlMX1 results in substantially increased drought tolerance and improved fruit quality, while knocking down SlMX1 resulted in the opposite phenotypes. Our study indicates an effective way with multiple beneficial traits by genetic engineering of a single regulatory gene and can be a novel approach to breeding crops.