Follistatin alters myostatin gene expression in C2C12 muscle cells
Follistatin alters myostatin gene expression in C2C12 muscle cells
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DOI：
10.1556/avet.52.2004.2.1
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发表时间：
2004-01-01
影响因子：
0.9
通讯作者：
Nazli, M
中科院分区：
文献类型：
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作者：
Kocamis, H;Gulmez, N;Nazli, M
本研究的目的是确定添加卵泡抑素对C2 C12肌细胞中myostatin和卵泡抑素基因表达模式的影响。每天向C2 C12细胞施用在含有10%胎牛血清（FBS）、4 mM谷氨酰胺和抗生素的Dulbecco改良Eagle培养基（DMEM）中的100 ng/ml重组人（rh）卵泡抑素，持续三天。在对照威尔斯孔中不加入Rh卵泡抑素。用逆转录聚合酶链反应（RTPCR）合成卵泡抑素和肌肉生长抑制素基因cDNA。卵泡抑素基因表达模式的时间过程在对照组和卵泡抑素治疗组中是相似的。在培养24 h后，卵泡抑素处理组的Myostatin mRNA水平显著增加（图3，P < 0.01）。然后在培养48小时时量急剧下降（图3，P < 0.01），而在培养72小时时对照组和卵泡抑素处理组之间没有显著差异。我们的研究结果表明，肌肉生长抑制素和卵泡抑素mRNA表达在C2 C12细胞和rhfollistatin改变肌肉生长抑制素的表达模式。
dThe objective of the present study was to determine the effects of follistatin addition on myostatin and follistatin gene expression patterns in C2C12 muscle cells. C2C12 cells were administered with 100 ng/ml recombinant human (rh) follistatin in Dulbecco's modified Eagle medium (DMEM) containing 10% fetal bovine serum (FBS), 4 mM glutamine and antibiotics daily for three days. Rh follistatin was not added in the control wells. Follistatin and myostatin gene cDNAs were synthesised by reverse transcriptase polymerase chain reaction (RTPCR). The time course of follistatin gene expression pattern was similar in both the control and the follistatin-treated group. Myostatin mRNA level significantly increased in the follistatin-treated group after 24 h of culture (Fig. 3, P < 0.01). Amounts then sharply decreased (Fig. 3, P < 0.01) at 48 h of culture, whereas there was no significant difference between the control and the follistatin-treated group at 72 h of culture. Our results demonstrated that myostatin and follistatin mRNA were expressed in C2C12 cells and rh follistatin changed the myostatin expression pattern.