Thyroid disruption effects of environmental level perfluorooctane sulfonates (PFOS) in Xenopus laevis
Thyroid disruption effects of environmental level perfluorooctane sulfonates (PFOS) in Xenopus laevis
复制标题
DOI：
10.1007/s10646-011-0749-3
复制
发表时间：
2011-11-01
期刊：
影响因子：
2.7
通讯作者：
Xie, Wen-ping
中科院分区：
文献类型：
--
作者：
Cheng, Yan;Cui, Yuan;Xie, Wen-ping
全氟辛烷磺酸（PFOS）是新兴的持久性有机污染物（POPs）之一，已引起国际社会日益广泛的关注，特别是与甲状腺系统发育和功能的潜在破坏有关。非洲爪蟾是一种两栖动物物种，广泛用作甲状腺破坏研究的合适两栖动物模型。为了研究环境低浓度PFOS暴露对甲状腺的干扰作用，将非洲小蝌蚪从46/47期到62期分别暴露于水中0.1、1、10和100μg/l的PFOS。结果表明，变态时间（以前肢羽化为代表，FLE）并没有随PFOS暴露而显着变化，而是呈现增加趋势（10μg/l除外）。曝光）。在 PFOS 暴露中观察到部分胶体消耗，但在治疗组中未观察到显着的组织学异常。此外，PFOS暴露导致甲状腺激素调节基因——甲状腺受体βA（TRβA）、碱性转录元件结合蛋白（BTEB）和II型脱碘酶（DI2）mRNA表达上调，呈现倒U形剂量反应模式。然而，与对照相比，III型脱碘酶（DI3）的mRNA表达并未受到影响。这些结果表明，PFOS 可能会扰乱非洲爪哇蝌蚪的甲状腺系统，导致 FLE 变化和甲状腺激素调节基因的调节交替。我们的研究提出了新的担忧，即环境相关水平下的 PFOS 可能会破坏两栖动物的甲状腺。
Perfluorooctane sulfonate (PFOS), one of the emerging persistent organic pollutants (POPs), has caused growing international concern especially related to the potential disruption in the development and function of thyroid system. Xenopus laevis is an amphibian species widely used as a suitable amphibian model for thyroid disruption research. To study the thyroid disruption effects related to PFOS exposure at environmental low levels, X. laevis tadpoles were exposed to 0.1, 1, 10 and 100 mu g/l PFOS in water respectively from stage 46/47 to stage 62. The results showed that the time to metamorphosis (presented by forelimb emergence, FLE) did not significantly change with PFOS exposure, but exhibited an increasing trend (except for 10 mu g/l exposure). Partial colloid depletion was observed for PFOS exposure, but no significant histological abnormality was observed in treatment groups. In addition, PFOS exposure resulted in up-regulation of thyroid hormone-regulated genes-thyroid receptor beta A (TR beta A), basic transcription element-binding protein (BTEB) and type II deiodinase (DI2) mRNA expression, presented as an inverted U-shaped dose response pattern. However, the mRNA expression of type III deiodinase (DI3) remained unaffected compared with the control. These results demonstrated that PFOS might disrupt the thyroid system in X. laevis tadpoles regarding FLE changes and regulation alternation of thyroid hormone-regulated genes. Our study has raised new concerns for possible thyroid disruption of PFOS in amphibians at environmental relevant levels.