Continuous irradiation with a 633-nm light-emitting diode exerts an anti-aging effect on human skin cells
Continuous irradiation with a 633-nm light-emitting diode exerts an anti-aging effect on human skin cells
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DOI：
10.3892/ijmm.2014.2030
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发表时间：
2015-02-01
影响因子：
5.4
通讯作者：
Park, Si Jun
中科院分区：
文献类型：
--
作者：
Kim, Hak Sun;Park, Won Sang;Park, Si Jun
越来越多的证据表明，发光二极管（LED）发出的光源对人体皮肤具有潜在的抗衰老作用。使用单次和间隔LED照射的研究已经证明了这种效果;然而，据我们所知，尚未研究连续LED照射的抗衰老效果。在本研究中，我们证明了633 +/- 3-nm LED的连续照射在体外和离体实验中都具有抗衰老作用。更具体地说，用633 nm LED照射2天增加了1型前胶原的合成，并降低了皮肤成纤维细胞中基质金属蛋白酶（MMP）1和MMP 2的表达。此外，用633 nm LED照射降低了炎症基因的表达水平，例如角质形成细胞中的环氧合酶-2（考克斯-2）和白细胞介素-1-α（IL-1 α）。此外，14天的LED照射适度增加角质形成细胞增殖。使用人类皮肤外植体，我们证实了这种633 nm LED照射的安全性，这导致了人体组织中的形态不变和无过敏潜力。总的来说，这些数据提供了对连续LED照射对人体皮肤的抗衰老作用的深入了解。
Accumulating evidence has indicated that the light source emitted from light-emitting diode (LED) has a potential anti-aging effect on human skin. Studies using single and interval LED irradiation have documented such effects; however, to the best of our knowledge, the anti-aging effects of continuous LED irradiation have not yet been investigated. In the present study, we demonstrated that continuous irradiation with a 633 +/- 3-nm LED exerted anti-aging effects in both in vitro and ex vivo experiments. More specifically, irradiation with a 633-nm LED for 2 days increased the synthesis of type 1 procollagen and decreased the expression of matrix metalloproteinase (MMP)1 and MMP2 in skin fibroblasts. In addition, irradiation with a 633-nm LED decreased the expression levels of inflammatory genes, such has cyclooxygenase-2 (COX-2), and interleukin-1-alpha (IL-1 alpha) in keratinocytes. Furthermore, a 14-day LED irradiation moderately increased keratinocyte proliferation. Using human skin explants, we confirmed the safety of this 633-nm LED irradiation, which resulted in unaltered morphology and allergy-free potential in human tissue. Overall, these data provide insight into the anti-aging effects of continuous LED irradiation on human skin.