The Trinodular Structure of Fibrinogen: Confirmation by both Shadowing and Negative Stain Electron Microscopy
The Trinodular Structure of Fibrinogen: Confirmation by both Shadowing and Negative Stain Electron Microscopy
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纤维蛋白原的三结结构：通过阴影和负染色电子显微镜确认
DOI：
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发表时间：
1979
影响因子：
6.7
通讯作者：
Harold P. Erickson
中科院分区：
文献类型：
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作者：
W. Fowler;Harold P. Erickson
纤维蛋白原分子喷在云母上，真空干燥后用铂遮蔽，呈现为三节棒状。我们在这里描述的负染色的浮选技术，我们能够可视化个别纤维蛋白原分子。在我们的负染色标本中，纤维蛋白原分子具有与阴影分子相同的三结节结构。分子长4 - 5 nm，两个外部结节直径为6-7 nm，中间结节直径为4-5 nm。我们认为，在以前的负染色研究中看到的直径为20 nm的小球是这些分子的聚集体。
Fibrinogen molecules sprayed on mica, dried in vacuo and shadowed with platinum appear as trinodular rods. We describe here the flotation technique of negative staining by which we were able to visualize individual fibrinogen molecules. The fibrinogen molecules in our negatively stained preparations have a trinodular structure identical to that of shadowed molecules. The molecules are 45 nm long, the two outer nodules are 6-7 nm in diameter, and the middle nodule is 4-5 nm in diameter. We believe that the 20 nm diameter globules seen in previous negative staining studies are aggregates of these molecules.