Serum cytochrome c level as a prognostic indicator in patients with systemic inflammatory response syndrome
Serum cytochrome c level as a prognostic indicator in patients with systemic inflammatory response syndrome
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DOI：
10.1016/j.cccn.2003.12.011
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发表时间：
2004-04-01
影响因子：
5
通讯作者：
Endo, F
中科院分区：
文献类型：
--
作者：
Adachi, N;Hirota, M;Endo, F
背景资料：细胞凋亡可能在全身炎症反应综合征（SIRS）的发生、发展为多器官功能障碍综合征（MODS）中起重要作用。为了量化这些疾病中细胞凋亡的程度，我们开发了一种夹心ELISA系统来测量血清细胞色素c（cyt-c）水平，并研究了SIRS/MODS患者中cyt-c浓度的预后意义。方法：对53例SIRS合并MODS或有MODS危险的患者和14例健康对照者进行血细胞色素C（Cyt-c）浓度测定。结果如下：SIRS/ MODS患者的Cyt-c浓度升高（0.24-210 ng/ml），而对照受试者的Cyt-c浓度低于检测限（0.1 ng/ml）。在入院当天和第5天，非幸存者的细胞色素C浓度与幸存者相比显著增加。cyt-c浓度与APACHE II和多器官功能衰竭（MOF）评分呈显著正相关。在急性加重期，Cyt-c浓度比MOF评分早升高，在存活者恢复期迅速下降，但在非存活者中，Cyt-c水平继续升高。结论：血清cyt-c水平的测定有助于评估STRS/MODS患者器官功能障碍的严重程度和预后。(C)2004 Elsevier B. V.保留所有权利。
Background: Apoptosis may play an important role in the development of systemic inflammatory response syndrome (SIRS) and progression to multiple organ dysfunction syndrome (MODS). To quantify the extent of apoptosis in these morbidities, we developed a sandwich ELISA system to measure serum cytochrome c (cyt-c) levels and we investigated the prognostic significance of cyt-c concentration in SIRS/MODS patients. Methods: Cyt-c concentrations in patients with SIRS (n=53) with or at risk for MODS were measured and compared with those of control subjects (n=14). Results: Cyt-c concentrations in SIRS/ MODS patients increased (0.24-210 ng/ml), whereas those in control subjects were under detection limits (0.1 ng/ml). Cyt-c concentrations in non-survivors increased significantly compared with those in survivors both on the day of admission and on the fifth hospital day. A significant positive correlation was found between cyt-c concentration and two representative organ dysfunction scores, APACHE II and multi-organ failure (MOF) score. Cyt-c concentrations increased earlier than MOF score during the exacerbation phase and rapidly decreased during the convalescence phase in a survivor, but the level continued to be high in a non-survivor. Conclusions: Determination of serum cyt-c concentrations may be useful to assess the severity of organ dysfunction and to predict the prognosis of STRS/MODS patients. (C) 2004 Elsevier B.V. All rights reserved.