Preparation of bionanocapsules by the layer-by-layer deposition of polypeptides onto a liposome
Preparation of bionanocapsules by the layer-by-layer deposition of polypeptides onto a liposome
复制标题
DOI：
10.1021/ma070477w
复制
发表时间：
2007-07-10
期刊：
影响因子：
5.5
通讯作者：
Fukui, Yuuka
中科院分区：
文献类型：
--
作者：
Fujimoto, Keiji;Toyoda, Tomonori;Fukui, Yuuka
以脂质体为模板制备了中空纳米粒子（生物纳米胶囊），用于生物聚合物的逐层沉积。我们将聚l -赖氨酸（P-Lys）和聚l -天冬氨酸（P-Asp）交替沉积在一种带负电荷的脂质体上，该脂质体由二脲酰磷脂酸（DLPA）和二肉豆酰磷脂酰胆碱（DMPC）制备而成。采用超滤技术实现了纳米胶囊与未结合聚合物的高效分离。每次沉积时纳米胶囊的ζ电位在正电荷和负电荷之间变化。FE-TEM显示脂质体表面覆盖有一层薄薄的聚合物层。将荧光探针1-羟基芘-3,6,8-三磺酸（HPTS）包封在纳米胶囊中。聚合物沉积抑制了HPTS的释放，并通过第一P-Lys层的覆盖率和DLPA的比例来调节释放速率。
Hollow nanoparticles (bionanocapsules) were prepared using liposomes as a template for the layer-by-layer deposition of biopolymers. We carried out the alternative deposition of poly-L-lysine (P-Lys) and poly-L-aspartic acid (P-Asp) onto a negatively charged liposome, which was prepared from dilauroyl phosphatidic acid (DLPA) and dimyristoylphosphatidylcholine (DMPC). Efficient separation of nanocapsules from unbound polymers was achieved by ultrafiltration. zeta-Potentials of nanocapsules changed between positive and negative charges at each deposition. FE-TEM revealed that the liposome was covered with a thin polymeric layer. A fluorescent probe, 1-hydroxypyrene-3,6,8-trisulfonic acid (HPTS), was encapsulated into nanocapsules. The release of HPTS was suppressed by the polymer deposition, and the release rate was tunable by coverage of the first P-Lys layer and the ratio of DLPA.