Experimental traction retinal detachment in the cat.
Experimental traction retinal detachment in the cat.
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猫实验性牵引性视网膜脱离。
DOI：
10.1007/bf00203323
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Machemer,R
中科院分区：
文献类型：
--
作者：
Wilson,CA;Khawly,JA;Hatchell,DL;Machemer,R
我们通过制造浆液性视网膜脱离，然后将2.5 × 105只小猫真皮成纤维细胞注射到视网膜伤口部位的玻璃体腔中，开发了一种可重复的猫眼牵引性视网膜脱离（TRD）模型。在静脉注射虎红（一种光敏染料）后，通过将视网膜区域暴露于聚焦光下产生浆液性视网膜脱离。TRD在成纤维细胞注射后的前2周内迅速发展，伴随着玻璃体视网膜链的形成，以及较小程度的视网膜前和/或视网膜下增殖。组织学检查显示玻璃体内或沿着玻璃体后表面有成纤维细胞。在视网膜内表面和/或视网膜下腔中偶尔发现成纤维细胞的局灶性沉积。通过放射性标记胸苷的摄取证实成纤维细胞增殖。早在成纤维细胞注射后3天就观察到胶原沉积。未观察到新血管形成。未接受成纤维细胞的对照眼显示浆液性脱离消退而无视网膜牵引。在所有眼睛中，在先前光动力治疗的区域中观察到视网膜变性和变薄。在该TRD模型中，前后牵引（由于玻璃体束）占主导地位，如在玻璃体内血液注射诱导的实验性后部穿透性眼损伤中观察到的，这也导致玻璃体束形成。然而，我们的模型能够在没有玻璃体血液产生的介质混浊的情况下对猫的TRD进行临床评估。哈切尔
We developed a reproducible model of traction retinal detachment (TRD) in the cat eye by creating a serous retinal detachment and then injecting 2.5 × 105kitten dermal fibroblasts into the vitreous cavity at the site of a retinal wound. Serous detachments were produced by exposing an area of retina to focused light after intravenous injection of rose bengal (a photosensitizing dye). TRD developed rapidly within the first 2 weeks after fibroblast injection, accompanied by the formation of vitreoretinal strands and, to a lesser degree, epiretinal and/or subretinal proliferation. Histopathology demonstrated fibroblasts within the vitreous or along the posterior hyaloid face. Focal deposits of fibroblasts were occasionally found on the inner surface of the retina and/or in the subretinal space. Fibroblast proliferation was confirmed by uptake of radiolabeled thymidine. Deposition of collagen was noted at as early as 3 days after fibroblast injection. Neovascularization was not observed. Control eyes that did not receive fibroblasts showed resolution of serous detachment without retinal traction. In all eyes, retinal degeneration and thinning were seen in the area of previous photodynamic treatment. In this model of TRD, anteroposterior traction (due to vitreous strands) predominates, as is observed in experimental posterior penetrating ocular injury induced by intravitreal blood injection, which also results in vitreous strand formation. Our model, however, enables clinical assessment of TRD in the cat without the media opacification produced by vitreous blood.Offprint requests to:D.L. Hatchell