The reversible inhibition of carbonic anhydrase II: Computer simulations of a proposed mechanism of action
The reversible inhibition of carbonic anhydrase II: Computer simulations of a proposed mechanism of action
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碳酸酐酶 II 的可逆抑制：所提出的作用机制的计算机模拟
DOI：
10.1007/bf01025173
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
R. Rowlett
中科院分区：
文献类型：
--
作者：
R. Rowlett
Steinine等人最初提出的碳酸酐酶II涉及限速分子内质子转移的作用机制，已经用数字计算机在稳态下完全重新研究。研究发现，该反应机理足以解释所有已有的动力学数据，包括外部缓冲液作为第二底物参与水合/脱水反应。Steineet等人的模型。已扩展到包括单价阴离子、苯酚和铜(II)离子的抑制作用，并能够在化学合理的框架内解释实验观察到的动力学。结论是，所提出的机理是碳酸氢酶II催化的一个很好的工作模型，它能够协调如此广泛的动力学数据，巩固了催化途径中限速分子内质子转移的概念。
A mechanism of action for carbonic anhydrase II involving a rate-limiting intramolecular proton transfer, originally proposed by Steineret al., has been reexamined totally in steady state form using a digital computer. It is found that the mechanism is sufficient to account for all of the extant kinetic data, including the participation of external buffer as a second substrate in the hydration/dehydration reaction. The model of Steineret al. has been expanded to include the inhibitory effects of monovalent anions, phenol, and Cu(II) ion, and is able to account for the experimentally observed kinetics within a chemically reasonable framework. It is concluded that the proposed mechanism is a good working model for carbonic anhydrase II catalysis, and its ability to reconcile such a wide body of kinetic data solidifies the notion of a rate-limiting intramolecular proton transfer in the catalytic pathway.
铜离子对人碳酸酐酶 II 催化的 18O 交换的抑制。
DOI：
--
发表时间：
1981
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Tu,C;Wynns,GC;Silverman,DN
通讯作者：
Silverman,DN