Type 1 and type 2 diabetic patients display different patterns of cellular microparticles
Type 1 and type 2 diabetic patients display different patterns of cellular microparticles
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DOI：
10.2337/diabetes.51.9.2840
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发表时间：
2002-09-01
期刊：
影响因子：
7.7
通讯作者：
Dignat-George, F
中科院分区：
文献类型：
--
作者：
Sabatier, F;Darmon, P;Dignat-George, F
糖尿病血管病变的发展涉及多因素过程，包括血管细胞的病理性激活。据报道，在与血栓形成风险相关的疾病中，激活的细胞释放微粒，但在糖尿病中几乎没有数据可用。本工作的目的是探索I型和2型糖尿病患者细胞来源的微粒的数量和促凝血活性。与年龄匹配的对照组相比，I型糖尿病患者的血小板和内皮细胞微粒子(PAH和EMP)、总Annexin V阳性血细胞微粒子(TMP)和TMP相关促凝活性水平显著增加。在2型糖尿病患者中，只有TMP水平显著升高，而他们的促凝血活性没有伴随增加。有趣的是，在1型糖尿病患者中，TMP促凝血活性与HbA(1c)相关，提示促凝血活性与葡萄糖失衡有关。这些结果表明，在糖尿病患者中，由于几种细胞类型的激活或凋亡，发生了广泛的囊泡形成过程。然而，糖尿病患者的促凝血活性和微粒的细胞来源不同。在I型糖尿病患者中，TIAP促凝血活性可能与血管并发症有关。此外，它与糖化血红蛋白(1c)的相关性，加强了1型糖尿病最佳血糖控制的重要性。
The development of vasculopathies in diabetes involves multifactorial processes including pathological activation of vascular cells. Release of microparticles by activated cells has been reported in diseases associated with thrombotic risk, but few data are available in diabetes. The aim of the present work was to explore the number and the procoagulant activity of cell-derived microparticles in type I and 2 diabetic patients. Compared with age-matched control subjects, type I diabetic patients presented significantly higher numbers of platelet and endothelial microparticles (PAH and EMP), total annexin V-positive blood cell microparticles (TMP), and increased levels of TMP-associated procoagulant activity. In type 2 diabetic patients, only TMP levels were significantly higher without concomitant increase of their procoagulant activity. Interestingly, in type 1 diabetic patients, TMP procoagulant activity was correlated with HbA(1c,) suggesting that procoagulant activity is associated with glucose imbalance. These results showed that a wide vesiculation process, resulting from activation or apoptosis of several cell types, occurs in diabetes. However, diabetic patients differ by the procoagulant activity and the cellular origin of microparticles. In type I diabetic patients, TIAP-procoagulant activity could be involved in vascular complications. Moreover, its correlation with HbA(1c), reinforces the importance of an optimal glycemic control in type 1 diabetes.