Tumor suppressor gene RBSP3 in cervical carcinoma:: Copy number and transcription level
Tumor suppressor gene RBSP3 in cervical carcinoma:: Copy number and transcription level
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DOI：
10.1134/s0026893307010116
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发表时间：
2007-02-01
影响因子：
1.2
通讯作者：
Senchenko, V. N.
中科院分区：
文献类型：
--
作者：
Anedchenko, E. A.;Kisseljova, N. P.;Senchenko, V. N.
Real-time PCR was used to quantitate structural and functional aberrations in the tumor suppressor gene RBSP3 (3p.21) in papillomavirus-positive cervical carcinoma (CC). RBSP3 deletions were found in 19 of 45 tumor specimens (42%). The frequency of deletions was reliably higher in tumors with metastases to regional lymph nodes (64%) than in tumors without metastases (32%, P < 0.05). In several cases, RBSP3 amplification was detected. Changes in RBSP3 copy number were observed in 51% (23/45) of the tumor specimens. The RBSP3 transcription level was decreased in 21 of 33 tumors (64%), this event being detected more frequently in tumors with (83%) than without metastases (52%, P < 0.05). In some tumors, RBSP3 mRNA levels were increased. Changes in RBSP3 mRNA level were observed in 79% (26/33) of the CCs. Comparative analysis of the data demonstrated that a decrease in RBSP3 mRNA level was a result of deletions only in 23% of the specimens, while it was not associated with any changes in the gene copy number in 36% of the cases. More unusual combinations of events were an unchanged or even increased mRNA level in the presence of deletions or an increased mRNA level in the absence of changes in gene copy number. It was assumed that deregulation of RBSP3 expression is associated with the progression of CC, that there is more than one mechanism of RBSP3 inactivation, and that RBSP3 has other functions in addition to pRb dephosphorylation.
