Optimization of in vitro propagation of Coleonema album, a highly utilized medicinal and ornamental plant
Optimization of in vitro propagation of Coleonema album, a highly utilized medicinal and ornamental plant
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DOI：
10.1016/j.sajb.2014.05.006
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发表时间：
2014-09
影响因子：
3.1
通讯作者：
O. O. Fajinmi-O.;S. Amoo;J. F. Finnie;J. Staden
中科院分区：
文献类型：
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作者：
O. O. Fajinmi-O.;S. Amoo;J. F. Finnie;J. Staden
Colonema albumis 是一种芳香、药用和观赏植物，在传统医药市场上需求量很大。为了满足日益增长的需求，同时保护野生这些种群，本研究旨在开发一种有效的 C.album 体外繁殖方案。研究了不同细胞分裂素[四间托普林与6-苄基腺嘌呤（BA）、激动素（KIN）和噻苯隆（TDZ）相比]对茎尖外植体直接器官发生的影响。每种细胞分裂素均以 2.5、5.0、7.5 和 10.0 μM 浓度进行测试。还研究了生长素与最佳细胞分裂素浓度的组合对芽增殖的影响。在等摩尔浓度下，与 KIN 相比，所有使用 BA、间托普林（mT，除 10 μM）、间托普林核苷和间甲氧基托普林四氢吡喃-2-基处理均显着增加了芽产量。芽产生的最佳细胞分裂素浓度为 5.0 μMmT，每个外植体再生 14.5 个芽。外源添加生长素进一步增加芽增殖（每个外植体最多 18 个芽）。研究结果表明，在本研究中，生长素与 mT 具有相加或协同作用。在补充了吲哚-3-丁酸（IBA）的半强度 Murashige 和 Skoog 培养基上生根的再生芽成功适应环境，存活率高于 70%。
Coleonema albumis an aromatic, medicinal and ornamental plant that is in high demand in the traditional medicine market. In order to meet the increasing demand while conserving those populations in the wild, this study was aimed at developing an efficientin vitropropagation protocol forC.album. The effect of different cytokinins [fourmeta-topolins in comparison with 6-benzyladenine (BA), kinetin (KIN) and thidiazuron (TDZ)] on direct organogenesis from shoot-tip explants was examined. Each cytokinin was tested at 2.5, 5.0, 7.5 and 10.0 μM. The effect of combining auxins with the optimal cytokinin concentration on shoot proliferation was also investigated. At equimolar concentrations, all treatments with BA,meta-topolin (mT, except 10 μM),meta-topolin riboside andmeta-methoxytopolin tetrahydropyran-2-yl significantly increased shoot production when compared to KIN. The optimum cytokinin concentration for shoot production was 5.0 μMmT with 14.5 shoots regenerated per explant. The exogenous addition of auxins further increased shoot proliferation (up to 18 shoots per explant). The findings suggest an additive or synergistic effect of auxin withmT in this study. Regenerated shoots rooted on half-strength Murashige and Skoog medium supplemented with indole-3-butyric acid (IBA) were successfully acclimatized with a survival frequency above 70%.