Polymer brushes showing non-fouling in blood plasma challenge the currently accepted design of protein resistant surfaces.
Polymer brushes showing non-fouling in blood plasma challenge the currently accepted design of protein resistant surfaces.
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DOI：
10.1002/marc.201100189
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发表时间：
2011-07
影响因子：
4.6
通讯作者：
César Rodriguez-Emmenegger;E. Brynda;T. Riedel;M. Houška;V. Šubr;A. Alles;E. Hasan;J. Gautrot
中科院分区：
文献类型：
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作者：
César Rodriguez-Emmenegger;E. Brynda;T. Riedel;M. Houška;V. Šubr;A. Alles;E. Hasan;J. Gautrot
首次合成了超低污垢聚N-（2-羟丙基）甲基丙烯酰胺（聚（HPMA））刷。与目前唯一的超低污染表面聚羧酸甜菜碱丙烯酰胺相似，血浆污染水平低于表面等离子体共振（SPR, 0.03 ng·cm(-2)）的检测极限，尽管它是氢键供体，并表现出中度的润湿性，从而挑战了目前公认的防污性能设计观点。即使经过两年的储存，其防污性能仍然保持不变。为了证明聚HPMA刷在制备生物活性超低污垢表面方面的潜力，制备了一种用于G链球菌检测的无标记SPR免疫传感器。
Ultra-low-fouling poly[N-(2-hydroxypropyl) methacrylamide] (poly(HPMA)) brushes have been synthesized for the first time. Similar to the so far only ultra-low-fouling surface, poly(carboxybetaine acrylamide), the level of blood plasma fouling was below the detection limit of surface plasmon resonance (SPR, 0.03 ng·cm(-2)) despite being a hydrogen bond donor and displaying a moderate wettability, thus challenging the currently accepted views for the design of antifouling properties. The antifouling properties were preserved even after two years of storage. To demonstrate the potential of poly(HPMA) brushes for the preparation of bioactive ultra-low fouling surfaces a label-free SPR immunosensor for detection of G Streptococcus was prepared.