Thalassotalea mangrovi sp. nov., a bacterium isolated from marine mangrove sediment
Thalassotalea mangrovi sp. nov., a bacterium isolated from marine mangrove sediment
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红树林海藻 (Thalassotalea mangrovi sp.)
DOI：
10.1099/ijsem.0.003677
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发表时间：
2019
影响因子：
2.8
通讯作者：
Xu Hong
中科院分区：
文献类型：
--
作者：
Zheng Sisi;Zhang Danyang;Gui Jiali;Wang Jiangning;Zhu Xiaoying;Lai Qiliang;Wang Wenqing;Xu Hong
从广西北仑河口国家级自然保护区的红树林湿地沉积物中分离到一株革兰氏染色阴性、严格需氧的细菌ZS-4 T，该细菌在指数生长期为杆状，具有单根极鞭毛，在稳定期为球形或卵形，无鞭毛。该菌株在pH 6.0-8.0、37 °C的温度和3- 4%（w/v）NaCl的存在下生长最佳。 其极性脂质谱包括磷脂酰乙醇胺、磷脂酰甘油、一种未鉴定的氨基磷脂和两种未表征的脂质。辅酶Q-8是唯一的呼吸醌，细胞脂肪酸以C17：1ω 8 c和C16：0为主。 16 S rRNA基因序列分析表明，菌株ZS-4 Te与模式菌株Thalassotalea litorea HMF 4135 T（97.8%）和Thalassotalea ponticola GJSW-36 T（95.9%）的相似性最高。 在全基因组水平上对菌株ZS-4 T和T. Litorea MCCC 1 K 03283 TCCC的平均核苷酸同一性为75.6%，计算的DNA-DNA杂交值为19.6%。 另外，菌株ZS-4 T基因组DNA的G+C含量为45.9mol%。根据形态学、生理生化、脂肪酸组成和16 S rRNA基因序列分析，菌株ZS-4 T应被认为是Thalassotalea属的一个新种，建议命名为Thalassotalea mangrovisp。模式菌株为ZS-4 T（=KCTC 72399 T =MCCC 1 K 03630 T）。
A novel Gram-stain-negative, strictly aerobic bacterium that has a rod-like shape with a single polar flagellum in the exponential phase of growth and a spherical or ovoid shape without a flagellum in the stationary phase was isolated from a mangrove wetland sediment sample collected at Beilun Estuary National Nature Reserve, Guangxi Province, PR China and designated strain ZS-4T. This strain grew optimally at pH 6.0–8.0, at a temperature of 37 °C and in the presence of 3–4 % (w/v) NaCl. Its polar lipid profile included phosphatidylethanolamine, phosphatidylglycerol, one unidentified aminophospholipid and two uncharacterized lipids. Ubiquinone 8 (Q-8) was the sole respiratory quinone and the cellular fatty acids were dominated by C17 : 1ω8cand C16 : 0. A phylogenetic analysis based on the 16S rRNA gene sequence showed that strain ZS-4Texhibited its highest similarities to the type strainsThalassotalea litoreaHMF4135T(97.8 %) andThalassotalea ponticolaGJSW-36T(95.9 %). A whole genome-level comparison of strain ZS-4TwithT. litoreaMCCC 1K03283Trevealed an average nucleotide identity value of 75.6 % and a calculated DNA–DNA hybridization value of 19.6 %. In addition, the genomic DNA G+C content of strain ZS-4Twas 45.9 mol%. Thus, based on analyses of its morphology, physiology, fatty acid composition and 16S rRNA gene sequence, strain ZS-4Tshould be considered a novel species of the genusThalassotalea, with the proposed nameThalassotalea mangrovisp. nov. The type strain is ZS-4T(=KCTC 72399T=MCCC 1K03630T).