Effect of the chemical composition of filter media on the microbial community in wastewater biofilms at different temperatures.
Effect of the chemical composition of filter media on the microbial community in wastewater biofilms at different temperatures.
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过滤器培养基在不同温度下废水生物膜中微生物群落的化学成分对微生物群落的影响。
DOI：
10.1039/c6ra21040f
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发表时间：
2016-11-01
期刊：
影响因子：
3.9
通讯作者：
Saroj DP
中科院分区：
文献类型：
--
作者：
Naz I;Hodgson D;Smith A;Marchesi J;Ahmed S;Avignone-Rossa C;Saroj DP
本研究采用下一代测序技术（NGS），对固定式生物膜反应器中两种不同载体上生长的生物膜中的微生物群落组成进行了研究。采用下一代测序（NGS）技术，研究了固定式生物膜反应器中两种不同载体上生长的生物膜中的微生物群落组成。发现新型支撑介质（TDR）的化学组成与常规使用的支撑介质（石头）有很大不同。从实验室规模的生物膜系统回收的16S rRNA基因片段的分析表明，生物膜支持介质和温度条件影响细菌群落结构和组成。在每种条件下观察到更大的细菌多样性，主要是由于大量的序列和稀有物种的维持。共发现6个门，其中变形菌门相对丰度最高（52.71%），其次是拟杆菌门（33.33%）、放线菌门（4.65%）、厚壁菌门、疣微菌门（3.1%）和绿软菌门（>1%）。该数据集显示在所有条件下共有17个属的细菌种群，表明在用于废水处理的生物膜中存在核心微生物群落。然而，在TDR上的生物膜中观察到高比例的一些属，这可能归因于其化学组成，解释了废水处理水平的提高。研究结果表明，生物膜系统中微生物群落结构受载体基质性质的影响。
This study investigates the microbial community composition, in the biofilms grown on two different support media in fixed biofilm reactors for aerobic wastewater treatment, using next generation sequencing (NGS) technology. This study investigates the microbial community composition in the biofilms grown on two different support media in fixed biofilm reactors for aerobic wastewater treatment, using next generation sequencing (NGS) technology. The chemical composition of the new type of support medium (TDR) was found to be quite different from the conventionally used support medium (stone). The analysis of 16S rRNA gene fragments recovered from the laboratory scale biofilm system show that biofilm support media and temperature conditions influence bacterial community structure and composition. Greater bacterial diversity was observed under each condition, primarily due to the large number of sequences available and sustenance of rare species. There were 6 phyla found, with the highest relative abundance shown by the phylum Proteobacteria (52.71%) followed by Bacteroidetes (33.33%), Actinobacteria (4.65%), Firmicutes, Verrucomicrobia (3.1%) and Chloroflex (>1%). The dataset showed 17 genera of bacterial populations to be commonly shared under all conditions, suggesting the presence of a core microbial community in the biofilms for wastewater treatment. However, some genera in the biofilms on TDR were observed in high proportions, which may be attributed to its chemical composition, explaining the improved level of wastewater treatment. The findings show that the structure of microbial communities in biofilm systems for wastewater treatment is affected by the properties of support matrix.
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影响因子：
5
作者：
Bargar, JR;Tebo, BM;Villinski, JE
通讯作者：
Villinski, JE
影响因子：
12.8
作者：
Ivnitsky, Hanan;Katz, Ilan;Dosoretz, Carlos G.
通讯作者：
Dosoretz, Carlos G.
DOI：
10.1007/s11783-011-0301-y
发表时间：
2011-03-01
影响因子：
--
作者：
He, Zhili;Van Nostrand, Joy D.;Zhou, Jizhong
通讯作者：
Zhou, Jizhong
DOI：
10.1099/00207713-51-2-527
发表时间：
2001-03-01
影响因子：
2.8
作者：
Achenbach, LA;Michaelidou, U;Coates, JD
通讯作者：
Coates, JD
影响因子：
2.7
作者：
Ginige, Maneesha P.;Wylie, Jason;Plumb, Jason
通讯作者：
Plumb, Jason