Application of ABTS radical cation for selective on-line detection of radical scavengers in HPLC eluates
Application of ABTS radical cation for selective on-line detection of radical scavengers in HPLC eluates
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DOI：
10.1021/ac0013610
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发表时间：
2001-07-15
影响因子：
7.4
通讯作者：
van Beek, TA
中科院分区：
文献类型：
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作者：
Koleva, II;Niederländer, HAG;van Beek, TA
2，2 ' -连氮双-（3 -乙基苯并噻唑啉-6-磺酸盐）阳离子自由基（ABTS（.+））用于在线HPLC方法检测复杂基质中的自由基清除剂。HPLC分离的分析物在柱后与预先形成的ABTS（.+）反应，在734 nm处有一个负吸收峰。该方法适用于使用含有100%有机溶剂或其水溶液、弱酸或缓冲液（pH 3-7.4）的移动的流动相的等度和梯度HPLC运行。该方法灵敏、选择性强、相对简单，适用于不同化学性质的化合物;使用常用仪器和廉价试剂;具有省时、省力的实验方案。它还可用于定量分析。该方法适用于几种纯天然抗氧化剂和植物提取物。最小可检测浓度从0.02到0.13 μ g/mL不等，取决于测试的化合物。该方法可以应用于进行动力学研究，这是通过测定Trolox等效抗氧化剂能力（TEAC）的几种已知的抗氧化剂在现在的注射模式。
The radical cation 2,2 ' -azinobis-(3 -ethylbenzothiazoline-6-sulfonate), (ABTS(.+)) was utilized in an on-line HPLC method for the detection of radical scavengers in complex matrixes. The HPLC-separated analytes react postcolumn with the preformed ABTS(.+), and the induced bleaching is detected as a negative peak by an absorbance detector at 734 nm, An optimized instrumental and experimental setup is presented. The method is suitable for both isocratic and gradient HPLC runs using mobile phases containing 100% organic solvent or,its solution in water, weak acids, or buffers (pH 3-7.4), The method is sensitive, selective, relatively simple, applicable to compounds of different chemical natures; uses common instruments and inexpensive reagents; and has a time-saving, non-laborious experimental protocol. It can also be used for quantitative analysis. The method was applied to several pure natural antioxidants and plant extracts. The minimum detectable concentration varied from 0.02 to 0.13 mug/mL, depending on the compound tested, The method can be applied to perform kinetic studies, which is illustrated by determination of Trolox equivalent antioxidant capacities (TEAC) of several known antioxidants in now injection mode.