The development and impact of 454 sequencing
The development and impact of 454 sequencing
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DOI：
10.1038/nbt1485
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发表时间：
2008-10-01
影响因子：
46.9
通讯作者：
Leamon, John H.
中科院分区：
文献类型：
--
作者：
Rothberg, Jonathan M.;Leamon, John H.
454测序仪极大地增加了科学界进行的测序量，并扩大了直接读取DNA序列可以解决的问题范围。454测序平台开发的关键突破包括更高的通量，简化了所有体外样品制备和测序化学的小型化，使大规模平行测序反应能够以前所未有的规模和成本进行。与其他最近发布的下一代技术一起，454平台已经开始使测序民主化，为各个实验室提供了与以前只有少数大型测序中心才能提供的能力相媲美的能力。在过去的18个月里，454测序使我们更好地了解了人类基因组的结构，允许第一个非Sanger序列的个体人类，并开辟了新的方法来识别小RNA。为了使下一代技术更广泛地获得，它们必须变得更容易使用和成本更低。从长远来看，454测序所建立的原则可能会进一步降低成本，从而可能实现个性化基因组学。
The 454 Sequencer has dramatically increased the volume of sequencing conducted by the scientific community and expanded the range of problems that can be addressed by the direct readouts of DNA sequence. Key breakthroughs in the development of the 454 sequencing platform included higher throughput, simplified all in vitro sample preparation and the miniaturization of sequencing chemistries, enabling massively parallel sequencing reactions to be carried out at a scale and cost not previously possible. Together with other recently released next-generation technologies, the 454 platform has started to democratize sequencing, providing individual laboratories with access to capacities that rival those previously found only at a handful of large sequencing centers. Over the past 18 months, 454 sequencing has led to a better understanding of the structure of the human genome, allowed the first non-Sanger sequence of an individual human and opened up new approaches to identify small RNAs. To make next-generation technologies more widely accessible, they must become easier to use and less costly. In the longer term, the principles established by 454 sequencing might reduce cost further, potentially enabling personalized genomics.