Disruption of actin cytoskeleton and anchorage-dependent cell spreading induces apoptotic death of mouse neural crest cells cultured in vitro
Disruption of actin cytoskeleton and anchorage-dependent cell spreading induces apoptotic death of mouse neural crest cells cultured in vitro
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DOI：
10.1002/ar.a.20150
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发表时间：
2005-02-01
期刊：
影响因子：
--
通讯作者：
Shiota, K
中科院分区：
文献类型：
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作者：
Hinoue, A;Takigawa, T;Shiota, K
在脊椎动物胚胎中，神经嵴细胞从神经管中迁移出来，促进各种神经和非神经组织的形成。一些神经嵴细胞在迁移过程中发生凋亡死亡，但其生物学意义和潜在机制尚不清楚。我们进行了一项体外研究，以研究当肌动蛋白细胞骨架或锚定依赖性细胞扩散受到干扰时，小鼠胚胎颅神经嵴（CNC）细胞的形态和存活如何受到影响。细胞松弛素D（1微克/毫升）破坏肌动蛋白纤维组织，基质金属蛋白酶-2 （MMP-2, 2.0单位/毫升）抑制细胞附着，随后培养的CNC细胞发生形态学改变和凋亡死亡。当肌动蛋白骨架被细胞松弛素D破坏时，培养的CNC细胞的形态变化先于DNA断裂。这些结果表明，维持细胞骨架和锚定依赖的细胞扩散是CNC细胞存活所必需的。蛋白酶的时空表达调控可能是神经嵴细胞分化和迁移的必要条件。(C) 2005 Wiley-Liss, Inc。
In vertebrate embryos, neural crest cells emigrate out of the neural tube and contribute to the formation of a variety of neural and nonneural tissues. Some neural crest cells undergo apoptotic death during migration, but its biological significance and the underlying mechanism are not well understood. We carried out an in vitro study to examine how the morphology and survival of cranial neural crest (CNC) cells of the mouse embryo are affected when their actin cytoskeleton or anchorage-dependent cell spreading is perturbed. Disruption of actin fiber organization by cytochalasin D (I mug/ml) and inhibition of cell attachment by matrix metalloproteinase-2 (MMP-2; 2.0 units/ml) were followed by morphologic changes and apoptotic death of cultured CNC cells. When the actin cytoskeleton was disrupted by cytochalasin D, the morphologic changes of cultured CNC cells preceded DNA fragmentation. These results indicate that the maintenance of cytoskeleton and anchorage-dependent cell spreading are required for survival of CNC cells. The spatially and temporally regulated expression of proteinases may be essential for the differentiation and migration of neural crest cells. (C) 2005 Wiley-Liss, Inc.