Resonance properties of different neuronal populations in the immature mouse neocortex
Resonance properties of different neuronal populations in the immature mouse neocortex
复制标题
DOI：
10.1111/j.1460-9568.2012.08196.x
复制
发表时间：
2012-09
影响因子：
3.4
通讯作者：
Haiyan Sun;H. Luhmann;W. Kilb
中科院分区：
文献类型：
--
作者：
Haiyan Sun;H. Luhmann;W. Kilb
在未成熟的新皮层中的体内记录揭示了从δ（0.5-4 Hz）到γ（30-100 Hz）频率的自发和感觉驱动的振荡活动。为了研究未成熟新皮层中不同神经元群体的共振特性是否有助于这些网络振荡，我们对出生后（P）0-P7 C57 Bl/6小鼠的切线和冠状新皮层切片中视觉识别的神经元进行了全细胞膜片钳记录。阈下谐振是通过注入频率变化的正弦电流来分析的。所有Cajal-Retzius细胞均显示出阈下共振，平均频率为2.6 ± 0.1 Hz（n = 60），ZD 7288（一种超极化激活阳离子电流阻断剂）可显著降低该频率。65.6%（n = 61）的颗粒上锥体神经元出现阈下共振，平均频率为1.4 ± 0.1Hz（n = 40）。应用Ni 2+抑制阈下共振，表明低阈值钙电流有助于这些神经元中的共振。约63.6%（n = 77）的V层锥体神经元显示阈下共振，平均频率为1.4 ± 0.2 Hz（n = 49），ZD 7288可消除该共振。仅44.1%（n = 59）的亚板神经元出现阈下共振，平均频率为1.3 ± 0.2Hz（n = 26），共振强度较小。总之，这些结果表明，所有研究层中的神经元均表现出共振行为，超极化激活的阳离子或低阈值钙电流有助于阈下共振。所观察到的共振频率是在未成熟的新皮层中观察到的缓慢活动模式的范围内，这表明阈下共振可能支持这种活动的产生。
In vivo recordings in the immature neocortex revealed spontaneous and sensory‐driven oscillatory activity from delta (0.5–4 Hz) to gamma (30–100 Hz) frequencies. In order to investigate whether the resonance properties of distinct neuronal populations in the immature neocortex contribute to these network oscillations, we performed whole‐cell patch‐clamp recordings from visually identified neurons in tangential and coronal neocortical slices from postnatal day (P)0–P7 C57Bl/6 mice. Subthreshold resonance was analysed by sinusoidal current injection of varying frequency. All Cajal–Retzius cells showed subthreshold resonance, with an average frequency of 2.6 ± 0.1 Hz (n = 60), which was massively reduced by ZD7288, a blocker of hyperpolarization‐activated cation currents. Approximately 65.6% (n = 61) of the supragranular pyramidal neurons showed subthreshold resonance, with an average frequency of 1.4 ± 0.1 Hz (n = 40). Application of Ni2+ suppressed subthreshold resonance, suggesting that low‐threshold calcium currents contribute to resonance in these neurons. Approximately 63.6% (n = 77) of the layer V pyramidal neurons showed subthreshold resonance, with an average frequency of 1.4 ± 0.2 Hz (n = 49), which was abolished by ZD7288. Only 44.1% (n = 59) of the subplate neurons showed subthreshold resonance, with an average frequency of 1.3 ± 0.2 Hz (n = 26) and a small resonance strength. In summary, these results demonstrate that neurons in all investigated layers show resonance behavior, with either hyperpolarization‐activated cation or low‐threshold calcium currents contributing to the subthreshold resonance. The observed resonance frequencies are in the range of slow activity patterns observed in the immature neocortex, suggesting that subthreshold resonance may support the generation of this activity.