Developmental appearance of the vitamin K-dependent protein of bone during calcification. Analysis of mineralizing tissues in human, calf, and rat.
Developmental appearance of the vitamin K-dependent protein of bone during calcification. Analysis of mineralizing tissues in human, calf, and rat.
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DOI：
10.1016/s0021-9258(19)69522-0
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发表时间：
1981-04
期刊：
影响因子：
--
通讯作者：
P. Price;J. W. Lothringer;S. Baukol;A. Reddi
中科院分区：
文献类型：
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作者：
P. Price;J. W. Lothringer;S. Baukol;A. Reddi
为了建立初始矿物质沉积和骨钙素出现之间的时间关系，已经分析了几种矿化组织的骨维生素K依赖蛋白（BGP）。这些研究表明，骨钙素在发育中的出现并不依赖于出生，在早期的大鼠发育的研究中已经提出，而是在先前的沉积骨矿物质。在胎儿人骨中，骨钙素的水平（骨钙素的克数/摩尔骨PO 4）从5%的成人水平在10周的胎龄上升到成人水平在15周。因此，骨钙素的成人水平达到人体骨骼后不久，最初出现的矿物质和出生前很长一段时间。在青春期大鼠中，骨中的BGP总体水平接近成人水平，在生长骨的末端和通过植入脱矿骨基质诱导的骨中的BGP出现在矿物质沉积之后约2周。骨钙素在最初沉积的骨矿物质中的相对缺乏及其随后几天后的出现可能与骨矿物质成熟为羟基磷灰石（一种结合骨钙素的结构）的因果关系有关。讨论了骨钙素在矿化组织中出现的时间对其在骨中可能的功能的影响。
Several mineralizing tissues have been analyzed for the vitamin K-dependent protein of bone (BGP) in order to establish the temporal relationship between initial mineral deposition and the appearance of BGP. These studies demonstrate that the appearance of BGP in developing bone is not dependent on birth, as had been suggested in earlier studies of rat development, but rather on the prior deposition of bone mineral. In fetal human bone, the level of BGP (grams of BGP/mol of bone PO4) rises from 5% of the adult level at 10 weeks gestational age to the adult level at 15 weeks. Thus, adult levels of BGP are reached in human bone shortly after the initial appearance of mineral and long before birth. In adolescent rats, which have overall levels of BGP in bone near the adult level, the appearance of BGP at the ends of growing bones and in bone induced by implantation of demineralized bone matrix follows mineral deposition by approximately 2 weeks. The relative absence of BGP in initially deposited bone mineral and its subsequent appearance several days later may be causally related to the maturation of bone mineral to hydroxyapatite, a structure which binds BGP. The implications of the timing of BGP appearance in mineralizing tissues to its possible function in bone are discussed.