Assessment of spatial distribution of growth in the elongation zone of grass leaf blades.
Assessment of spatial distribution of growth in the elongation zone of grass leaf blades.
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DOI：
10.1104/pp.85.1.290
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发表时间：
1987-09
期刊：
影响因子：
7.4
通讯作者：
H. Schnyder;C. J. Nelson;J. H. Coutts
中科院分区：
文献类型：
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作者：
H. Schnyder;C. J. Nelson;J. H. Coutts
关于生长的空间分布的知识对于理解叶的生长过程是必不可少的。在禾本科植物中，伸长区位于叶片的底部，并被老叶的叶鞘包围。因此，对空间生长分布的评估需要使用破坏性方法。我们用细针在高羊茅(Festuca arundinacea Schreb.)叶片伸长区的分蘖根部打孔，然后在间隔6或24小时后测量打孔的位移。针孔导致每日叶片伸长下降22%至41%，因此进行了试验，以研究伸长区生长的空间分布是否发生了变化。当针孔在发生细胞伸长的区域之内或以上时，叶片伸长速率也同样降低。通过去除周围组织后放置在延长区表皮上的针孔或墨迹的移位来估计延长区内的延伸率分布是相同的。在伸长区内不同位置打孔对叶片伸长的相对贡献率无显著差异。这些发现表明，成对叶片上的针孔或墨迹可以用来估计未受损害的高羊茅叶片伸长区生长的相对分布。
Knowledge about the spatial distribution of growth is essential for understanding the leaf growth process. In grasses the elongation zone is located at the base of the leaf blade and is enclosed by sheaths of older leaves. Assessment of spatial growth distribution, therefore, necessitates use of a destructive method. We used a fine needle to make holes through bases of tillers at the location of the leaf elongation zone of tall fescue (Festuca arundinacea Schreb.), then measured the displacement of the holes after a 6 or 24 h interval. Needle holes caused a 22 to 41% decrease in daily leaf elongation so experiments were conducted to investigate if the spatial distribution of growth in the elongation zone was altered. Leaf elongation rate was reduced similarly when needle holes were made within or above the zone where cell elongation occurs. Distribution of elongation within the zone was the same when estimated by displacement of needle holes or ink marks placed on the epidermis of the elongation zone after surrounding tissue had been removed. Making holes at different locations within the elongation zone did not differentially affect the relative contribution of the damaged or undamaged parts to leaf elongation. These findings demonstrate that needle holes or ink marks in paired leaves can be used to estimate the relative distribution of growth in the elongation zone of undamaged tall fescue leaf blades.