MECHANISMS OF RESISTANCE OF WHOLE CELLS TO TOXIC ORGANIC-SOLVENTS
MECHANISMS OF RESISTANCE OF WHOLE CELLS TO TOXIC ORGANIC-SOLVENTS
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DOI：
10.1016/0167-7799(94)90029-9
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发表时间：
1994-10-01
影响因子：
17.3
通讯作者：
DEBONT, JAM
中科院分区：
文献类型：
--
作者：
HEIPIEPER, HJ;WEBER, FJ;DEBONT, JAM
现代生物技术中的许多过程，特别是生物转化和环境生物修复，受到有机溶剂对整个细胞的毒性作用的阻碍。这些化合物溶解在细胞膜中，破坏其完整性并影响特定的透化作用。化合物的疏水性，以其log P值表示，是毒性的良好指标。log P值在1-5范围内的物质通常对全细胞有毒。然而，近年来，有几个报道细菌表现出对有毒溶剂的抗性。主要的适应性反应是膜组成的改变，特别是脂肪酸组成、磷脂头基和蛋白质含量的变化。使一些假单胞菌菌株适应有机溶剂的关键过程之一似乎是顺式不饱和脂肪酸异构化为反式不饱和脂肪酸。对这些适应性的更深入理解最终将使生物转化反应在包含有机相的不适宜的两相系统中进行。
Many processes in modern biotechnology, particularly biotransformations and environmental bioremediation, are hindered by the toxic effects of organic solvents on whole cells. These compounds dissolve in the cell membrane, disturbing its integrity and effecting specific permeabilization. The hydrophobicity of a compound, expressed as its log P value, is a good indicator of toxicity. Substances with a log P value in the range 1-5 are, in general, toxic to whole cells. However, in recent years, there have been several reports of bacteria exhibiting resistance to toxic solvents. The main adaptative reactions are alterations in the composition of the membrane, particularly changes in fatty-acid composition, phospholipid headgroups, and in the protein content. One of the key processes in the adaptation of some Pseudomonas strains, enabling them to tolerate organic solvents appears to be the isomerization of cis- into trans-unsaturated fatty acids. A greater understanding of these adaptations should eventually allow biotransformation reactions to be carried out in inhospitable two-phase systems incorporating an organic phase.