Effective display of metallothionein tandem repeats on the bioadsorption of cadmium ion
Effective display of metallothionein tandem repeats on the bioadsorption of cadmium ion
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DOI：
10.1007/s00253-005-0093-8
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发表时间：
2006-04-01
影响因子：
5
通讯作者：
Ueda, M
中科院分区：
文献类型：
--
作者：
Kuroda, K;Ueda, M
为了提高表面工程酵母中重金属离子的吸附水平，通过基于alpha-agglutinin的显示系统进行融合并显示酵母金属硫硫代蛋白（YMT）。通过免疫荧光标记检查了YMT及其串联重复的显示。随着串联重复量的增加，CD2+在细胞表面上的吸附和回收率越来越大。 YMT串联重复序列中的所有CD2+结合位点都被完全占据。为了研究细胞表面的吸附和针对重金属离子毒性的保护，通过在CD2+含有含CD2+的液体培养基中生长来检查这些表面工程酵母对CD2+的耐受性。发现生长速率取决于YMT显示的串联重复序列的数量。这些结果表明，表面工程酵母作为生物助剂的特征取决于显示的蛋白质结合金属离子的能力，并且重金属离子在细胞表面上的吸附在细胞表面的能力中起着重要作用。抵抗金属离子的毒性作用。
To increase the level of adsorption of heavy metal ions in surface-engineered yeasts, a yeast metallothionein (YMT) was tandemly fused and displayed by means of an alpha-agglutinin-based display system. The display of the YMT and its tandem repeats was examined by immunofluorescent labeling. The adsorption and recovery of Cd2+ on the cell surface was increasingly enhanced with increasing number of tandem repeats. All Cd2+-binding sites in the YMT tandem repeats were suggested to be completely occupied. To investigate the relationship between cell-surface adsorption and protection against heavy metal ion toxicity, the tolerance of these surface-engineered yeasts to Cd2+ was examined by growing in Cd2+-containing liquid medium. The rate of growth was found to be dependent on the number of displayed tandem repeats of YMT. These results suggest that the characteristics of surface-engineered yeasts as a bioadsorbent were dependent on the ability of the displayed proteins to bind metal ions, and the adsorption of heavy metal ions on the cell surface plays a major role in the ability of the cells to resist the toxic effects of metal ions.