A sandwich-type label-free electrochemiluminescence immunosensor for neurotensin based on sombrero model with graphene-hyaluronate-luminol composite
A sandwich-type label-free electrochemiluminescence immunosensor for neurotensin based on sombrero model with graphene-hyaluronate-luminol composite
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DOI：
10.1016/j.electacta.2014.05.039
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发表时间：
2014-07
影响因子：
6.6
通讯作者：
Zhiyong Guo;Shuping Du;Beibei Chen;Yuhong Sha;Bin Qiu;Xiaohua Jiang;Sui Wang;Xing Li
中科院分区：
文献类型：
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作者：
Zhiyong Guo;Shuping Du;Beibei Chen;Yuhong Sha;Bin Qiu;Xiaohua Jiang;Sui Wang;Xing Li
开发了一种基于草帽模型的超灵敏夹心型无标记电化学发光（ECL）免疫传感器，用于检测人血清和尿液中的神经降压素（NT）。将石墨烯-透明质酸-鲁米诺（G-HY-鲁米诺）复合物涂覆在玻碳电极表面构建ECL免疫传感器，通过鲁米诺发出原位ECL信号。使用二抗 NT 未标记抗体形成最终的夹心免疫复合物作为阔边帽模型，有利于降低 ECL 强度，显着提高检测灵敏度。结果表明，ECL 的抑制强度随着 NT 浓度的对数而增加，在 0.001 pg cm−3 至 100 pg cm−3 的较宽线性范围内。此外，特异性、稳定性、重现性、再生性和应用均令人满意。因此，这种开发的ECL免疫传感器具有在临床诊断中实际检测小分子量NT和NT样肽的潜力。
An ultrasensitive sandwich-type label-free electrochemiluminescence (ECL) immunosensor based on sombrero model for the detection of neurotensin (NT) in human serum and urine was developed. Graphene-hyaluronate-luminol (G-HY-luminol) composite was coated on the surface of the glassy carbon electrode to build the ECL immunosensor, which gave in-situ ECL signals by luminol. A secondary NT unlabelled antibody was used to form the final sandwich immunocomplex as a sombrero model, facilitating reduction of the ECL intensity to improve the sensitivity of detection significantly. Results showed that the inhibition of ECL intensity increased with the logarithm of NT concentration within a wide linear range from 0.001 pg cm−3to 100 pg cm−3. In addition, specificity, stability, reproducibility, regeneration and application were satisfactory. Therefore, this developed ECL immunosensor has a potential for practical detection of NT and NT-like peptides with small molecular weight in the clinical diagnostics.