Effect of charge density of polysaccharide on self-assembly behaviors of ovalbumin and sodium alginate
Effect of charge density of polysaccharide on self-assembly behaviors of ovalbumin and sodium alginate
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多糖电荷密度对卵清蛋白和海藻酸钠自组装行为的影响
DOI：
10.1016/j.ijbiomac.2019.10.279
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发表时间：
2020-07-01
影响因子：
8.2
通讯作者：
Cheng, Shuiyuan
中科院分区：
文献类型：
--
作者：
Li, Junzhu;Jin, Weiping;Cheng, Shuiyuan
研究了卵清蛋白（OVA）与两种不同电荷密度（λ（SA 1）：λ（SA 2）约为2：1）的海藻酸钠（SA 1和SA 2）组装的异同。通过相图、粒径和微观结构表征了OVA/SA混合物的组装过程。OVA/SA 1和OVA/SA 2混合物在相图中明显观察到两个差异。首先，由于SA 1的较高电荷密度，OVA和SA 1之间的强相互作用导致仅记录pH（phi 1）。SA 1的较高线性电荷密度使pH（phi 1）-pH（phi 2）范围在2：1和1：1的比率下变窄。第二，OVA/SA 1复合物形成凝聚层，对离子诱导的屏蔽效应具有较强的抵抗力。这在没有250 mM NaCl的凝聚层中保持了更小的尺寸（更紧密的结构）和更大数量的复合物。具有不同电荷密度的调节性多糖可以控制复合物的溶解区域，并赋予蛋白质和多糖组装的凝聚体不同的尺寸或形态。(C)2019 Elsevier B. V.版权所有。
Similarities and differences of assembly for ovalbumin (OVA) and two kinds of sodium alginate (SA1 and SA2) varying in charge densities (lambda(SA1): lambda(SA2) approximate to 2:1) were investigated. The assembly processes of OVA/SA mixtures were characterized by phase diagram, particle size, and microstructure. Two differences between OVA/SA1 and OVA/SA2 mixtures in the phase diagram were distinctly observed. First, due to the higher charge density of SA1, the strong interaction between OVA and SA1 caused only pH(phi 1) to be recorded. A higher linear charge density of SA1 narrowed the pH(phi 1)-pH(phi 2) range at ratios of 2:1 and 1:1. Second, OVA/SA1 complexes formed a coacervate with a relatively strong resistance to ion-induced shielding effects. This maintained the smaller size (tighter structure) with a larger number of complexes in the coacervate without 250 mM NaCl. The regulating polysaccharides with different charge densities could control the soluble region of complexes and endow various size or morphology of the coacervate assembled by proteins and polysaccharides. (C) 2019 Elsevier B.V. All rights reserved.