Characterization of mRNA and genes for aldose reductase in rat.
Characterization of mRNA and genes for aldose reductase in rat.
复制标题
大鼠醛糖还原酶 mRNA 和基因的表征。
DOI：
10.1016/s0006-291x(88)81335-4
复制
发表时间：
1988
影响因子：
3.1
通讯作者：
D. Carper
中科院分区：
文献类型：
--
作者：
C. Nishimura;C. Graham;T. Hohman;M. Nagata;W. Robison;D. Carper
醛糖还原酶（AR；E.C. 1. 1. 1. 21）与多种糖尿病并发症有关。为了研究这种酶在易受此类并发症影响的靶组织中的表达，通过 Northern blot 杂交在各种组织和培养的细胞制剂中对编码 AR 的 mRNA 进行了表征。 AR mRNA 的大小（~1500 个碱基）与序列分析确定的大小非常一致。来自大鼠晶状体的 AR cDNA 探针与从培养的狗晶状体上皮细胞、培养的人视网膜毛细血管周细胞（壁细胞）和 Y 79 人视网膜母细胞瘤细胞分离的相同大小的 RNA 杂交。在大鼠组织中，大量的mRNA不仅在晶状体中表达，还在视网膜、坐骨神经和肾髓质中表达。 AR mRNA 在大鼠骨骼肌和大脑中似乎含量较低，并且在肝脏中几乎不存在。此外，大鼠基因组DNA的Southern印迹分析表明存在多个与AR相关的序列，可能表明多基因家族的存在。
Aldose reductase (AR; E.C. 1. 1. 1. 21) has been implicated in a variety of diabetic complications. To investigate the expression of this enzyme in target tissues susceptible to such complications, mRNA encoding AR was characterized by Northern blot hybridization in various tissues and cultured cell preparations. The size of mRNA for AR (∼1500 bases) was in good agreement with the size determined by sequence analysis. A cDNA probe for AR from rat lens hybridized to the same size species of RNA isolated from cultured dog lens epithelial cells, cultured human retinal capillary pericytes (mural cells), and Y 79 human retinoblastoma cells. In rat tissues, a substantial amount of mRNA was expressed not only in lens, but also in retina, sciatic nerve and kidney medulla. AR mRNA seemed to be less abundant in rat skeletal muscle and brain, and was scarcely present in liver. Furthermore, Southern blot analysis of rat genomic DNA indicated that there are multiple sequences related to that for AR, probably indicating the existence of a multi-gene family.
索宾尼尔（一种醛糖还原酶抑制剂）可减少糖尿病患者的蛋白尿。
影响因子：
3.1
作者：
Beyer-Mears,A;Cruz,E;Edelist,T;Varagiannis,E
通讯作者：
Varagiannis,E