CHARACTERIZATION OF NEUTRAL GLYCOSPHINGOLIPIDS IN RAT LENS
CHARACTERIZATION OF NEUTRAL GLYCOSPHINGOLIPIDS IN RAT LENS
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DOI：
10.1016/s0014-4835(95)80010-7
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发表时间：
1995-02-01
影响因子：
3.4
通讯作者：
KOMOTO, M
中科院分区：
文献类型：
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作者：
OGISO, M;OHTA, M;KOMOTO, M
中性鞘糖脂纯化非白内障的Sprague-Dawley大鼠晶状体的溶剂萃取，Folch的分配，并使用DEAE-Sephadex和latrobeads柱层析的组合。通过糖组成分析、甲基化分析和糖苷酶消化鉴定了从单己糖神经酰胺到五己糖神经酰胺的6个主要GSL。六种中性鞘糖脂之间的结构关系揭示了导致Gal α 1-3Gal β 1-4GlcNAc β 1-3Gal β 1 - 4Glc β 1- 1神经酰胺（IV（3）Gal α nLC 4）而不是刘易斯（x）糖脂合成的代谢途径（Gal β 1-4（Fuc α 1-3）GlcNAc β 1-3Gal β 1-4Glc β 1- 1神经酰胺，III（3）FucnLc 4），来自新乳四糖基神经酰胺（nLc 4），以及异葡萄糖三糖基神经酰胺（iGb 3）。α-半乳糖基表位Gal α 1-3Gal-R在非灵长类哺乳动物、原猴和新世界猴的许多类型的细胞中进化上保守，但在旧世界猴或人类的细胞中不保守。这种进化相关的碳水化合物表位的差异表明不同的细胞与细胞的连接，这可能是通过细胞表面鞘糖脂介导的，大鼠和人类之间的晶体。
Neutral glycosphingolipids were purified from non-cataractous lenses of Sprague-Dawley rats by a combination of solvent extraction, Folch's partition, and column chromatography using DEAE-Sephadex and latrobeads. Six major GSLs from monohexosylceramide to pentahexosylceramide were identified by sugar composition analysis, methylation analysis and glycosidase digestion. Structural relationships among the six neutral glycosphingolipids revealed metabolic pathways leading to the synthesis of Gal alpha 1-3Gal beta 1-4GlcNAc beta 1-3Gal beta 1-4Glc beta 1-1ceramide (IV(3)Gal alpha nLC4), instead of a Lewis(x) glycolipid (Gal beta 1-4(Fuc alpha 1-3)GlcNAc beta 1-3Gal beta 1-4Glc beta 1-1ceramide, III(3)FucnLc4), from neolactotetraosylceramide (nLc4), together with isoglobotriaosylceramide (iGb3). The alpha-galactosyl epitope, Gal alpha 1-3Gal-R, is evolutionarily conserved in many types of cells of non-primate mammals, prosimians and New World monkeys, but not in those of Old World monkeys or humans. This evolution-related difference in carbohydrate epitopes suggests different cell-to-cell attachments, which may be mediated through cell surface glycosphingolipids, between rat and human lenses.