Synergistic Interaction between Candida albicans and Commensal Oral Streptococci in a Novel In Vitro Mucosal Model
Synergistic Interaction between Candida albicans and Commensal Oral Streptococci in a Novel In Vitro Mucosal Model
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DOI：
10.1128/iai.05896-11
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发表时间：
2012-02-01
影响因子：
3.1
通讯作者：
Dongari-Bagtzoglou, Anna
中科院分区：
文献类型：
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作者：
Diaz, Patricia I.;Xie, Zhihong;Dongari-Bagtzoglou, Anna
白色念珠菌是人类胃肠道的肠道定殖者，在那里它与高度多样化的细菌群落共存。目前尚不清楚这种相互作用是否限制或促进了C的潜力。白色念珠菌成为机会致病菌。在这里，我们研究了C。白色念珠菌和来自草绿色菌群的三种链球菌，它们是普遍存在且丰富的口腔粘膜细菌。C.使用允许在唾液流下形成非生物膜的流动池装置研究了白色念珠菌与口腔链球菌、血链球菌或戈登链球菌形成生物膜的能力。此外，我们设计了一种新型的流动池系统，允许在模拟口腔和食管粘膜环境的条件下形成粘膜生物膜。结果表明，C.白色念珠菌和链球菌形成协同伙伴关系，其中C.白色念珠菌促进链球菌在非生物表面或口腔粘膜类似物表面上形成生物膜的能力。链球菌在C.白色念珠菌不能用生长刺激效应来解释，因为链球菌在与C.白色念珠菌相反，链球菌的存在增加了C。在唾液流的条件下，白色念珠菌侵入口腔和食管粘膜的器官型模型。此外，生物膜微生物对粘膜侵袭的表征表明食管粘膜比口腔粘膜更容易侵袭。综上所述，C.白色念珠菌和口腔链球菌显示出协同作用，暗示了念珠菌的致病潜力。白色念珠菌在上消化道。
Candida albicans is a commensal colonizer of the gastrointestinal tract of humans, where it coexists with highly diverse bacterial communities. It is not clear whether this interaction limits or promotes the potential of C. albicans to become an opportunistic pathogen. Here we investigate the interaction between C. albicans and three species of streptococci from the viridans group, which are ubiquitous and abundant oral commensal bacteria. The ability of C. albicans to form biofilms with Streptococcus orals, Streptococcus sanguinis, or Streptococcus gordonii was investigated using flow cell devices that allow abiotic biofilm formation under salivary flow. In addition, we designed a novel flow cell system that allows mucosal biofilm formation under conditions that mimic the environment in the oral and esophageal mucosae. It was observed that C. albicans and streptococci formed a synergistic partnership where C. albicans promoted the ability of streptococci to form biofilms on abiotic surfaces or on the surface of an oral mucosa analogue. The increased ability of streptococci to form biofilms in the presence of C. albicans could not be explained by a growth-stimulatory effect since the streptococci were unaffected in their growth in planktonic coculture with C. albicans. Conversely, the presence of streptococci increased the ability of C. albicans to invade organotypic models of the oral and esophageal mucosae under conditions of salivary flow. Moreover, characterization of mucosal invasion by the biofilm microorganisms suggested that the esophageal mucosa is more permissive to invasion than the oral mucosa. In summary, C. albicans and commensal oral streptococci display a synergistic interaction with implications for the pathogenic potential of C. albicans in the upper gastrointestinal tract.