DETERMINATION OF IODINE AND BROMINE IN PLASMA AND URINE BY INDUCTIVELY COUPLED PLASMA MASS-SPECTROMETRY
DETERMINATION OF IODINE AND BROMINE IN PLASMA AND URINE BY INDUCTIVELY COUPLED PLASMA MASS-SPECTROMETRY
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DOI：
10.1039/an9901500813
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发表时间：
1990-06-01
期刊：
影响因子：
4.2
通讯作者：
BEAUGRAND, C
中科院分区：
文献类型：
--
作者：
ALLAIN, P;MAURAS, Y;BEAUGRAND, C
描述了使用 Nermag 原型仪器通过电感耦合等离子体质谱法同时测定血浆和尿液中的碘和溴。样品制备仅涉及使用含有铕作为内标的稀释剂稀释 10 倍，然后直接雾化到血浆中。碘、溴和铕离子分别在 m/z= 127、79 和 153 处测量。该方法的灵敏度对于碘和溴的检测限分别为 1.6 和 52 µg l-1，满足临床应用的要求。碘和溴的校准图分别在 0–400 µg l–1 和 0–40 mg l–1 范围内呈线性，对于大多数测定而言，该范围足够宽。回收率接近 100%，变异系数小于 3%。日内和日间重现性约为 5%。 26 名健康个体血浆中碘和溴的浓度分别为 58 ± 12 µg l-1 和 4.1 ± 0.9 mg l-1。尿液中消除的碘和溴量分别为每 24 小时 94 ± 97 µg（范围为每 24 小时 27–403 µg）和每 24 小时 3.6 ± 1.7 mg。这些结果与报告值一致。
The simultaneous determination of iodine and bromine in plasma and urine by inductively coupled plasma mass spectrometry, using a Nermag prototype instrument, is described. The sample preparation involves only a 10-fold dilution with a diluent containing europium as an internal standard followed by direct nebulisation in the plasma. The iodine, bromine and europium ions are measured at m/z= 127, 79, and 153, respectively. The sensitivity of the method, with detection limits of 1.6 and 52 µg l–1 for iodine and bromine, respectively, is satisfactory for clinical applications. The calibration graphs were linear over the ranges 0–400 µg l–1 and 0–40 mg l–1 for iodine and bromine, respectively, which are wide enough for most assays. The recoveries were close to 100% with coefficients of variation of less than 3%. The within-day and between-day reproducibility was about 5%. The concentrations of iodine and bromine in the plasma of 26 healthy individuals were 58 ± 12 µg l–1 and 4.1 ± 0.9 mg l–1, respectively. The amounts of iodine and bromine eliminated in urine were 94 ± 97 µg per 24 h (range 27–403 µg per 24 h) and 3.6 ± 1.7 mg per 24 h, respectively. These results are in agreement with reported values.