Ballooned hepatocytes in steatohepatitis: The value of keratin immunohistochemistry for diagnosis
Ballooned hepatocytes in steatohepatitis: The value of keratin immunohistochemistry for diagnosis
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DOI：
10.1016/j.jhep.2008.01.026
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发表时间：
2008-05-01
影响因子：
25.7
通讯作者：
Denk, Helmut
中科院分区：
文献类型：
--
作者：
Lackner, Carolin;Gogg-Kamerer, Margit;Denk, Helmut
背景/目的：肝细胞“膨胀”是肝脏病理学中经常使用但定义不明确的术语，指与细胞肿胀和增大相关的特殊形式的肝细胞变性，特别是在脂肪性肝炎中。肝细胞中间丝细胞骨架的改变可能有助于这种微观变化的发病机制。气球样变性被认为是脂肪性肝炎的一个标志，但在多种其他急性和慢性肝脏疾病中也可能观察到肝细胞增大。方法：使用角蛋白 8 和 18 免疫组织化学研究与肝细胞增大或气球样变相关的肝脏疾病中的中间丝细胞骨架。结果：角蛋白 酒精性和非酒精性脂肪性肝炎、慢性胆汁淤积性疾病、缺血/再灌注损伤中的气球状肝细胞的细胞质以及并发脂肪性肝炎的慢性丙型肝炎病例中的气球状肝细胞的细胞质中的8/18免疫染色急剧减少或丢失。相比之下，在急性肝炎、巨细胞肝炎、慢性乙型肝炎或自身免疫性肝炎的病例中，没有观察到角蛋白8/18免疫染色的显着减少或丧失。结论：角蛋白8/18免疫染色的丧失可以作为特定类型肝细胞气球样变性的客观标志物。氧化应激可能是这些条件下角蛋白丝改变发病机制的共同点。 (C) 2008 年欧洲肝脏研究协会。由 Elsevier B.V. 出版。保留所有权利。
Background/Aims: Hepatocyte "ballooning" is an often used but ill defined term in liver pathology to designate a special form of liver cell degeneration associated with cell swelling and enlargement found particularly in steatohepatitis. Alterations of the intermediate filament cytoskeleton of the hepatocyte may contribute to the pathogenesis of this microscopic change. Ballooning degeneration is considered a hallmark of steatohepatitis, but enlarged hepatocytes may also be observed in a variety of other acute and chronic liver diseases.Methods: The intermediate filament cytoskeleton was investigated using keratin 8 and 18 immunohistochemistry in liver diseases associated with enlarged or ballooned hepatocytes.Results: Keratin 8/18 immunostaining was drastically reduced or lost in the cytoplasm of ballooned hepatocytes in alcoholic and non-alcoholic steatohepatitis, chronic cholestatic conditions, ischemia/reperfusion injury and in ballooned hepatocytes in chronic hepatitis C cases with concurrent steatohepatitis. In contrast, substantial decrease or loss of keratin 8/18 immunostaining was not noted in cases of acute hepatitis, giant cell hepatitis, chronic hepatitis B, or autoimmune hepatitis.Conclusions: Loss of keratin 8/18 immunostaining can serve as an objective marker of a specific type of ballooning degeneration of hepatocytes. Oxidative stress may be a common denominator in the p athogenesis of keratin filament alterations in these conditions. (C) 2008 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved.