Cytokine Production and Cytotoxicity of Calcium Silicate-based Sealers in 2-and 3-dimensional Cell Culture Models
Cytokine Production and Cytotoxicity of Calcium Silicate-based Sealers in 2-and 3-dimensional Cell Culture Models
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DOI：
10.1016/j.joen.2020.03.011
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发表时间：
2020-06-01
影响因子：
4.2
通讯作者：
Peters, Ove Andreas
中科院分区：
文献类型：
--
作者：
Gaudin, Alexis;Tolar, Mirek;Peters, Ove Andreas
简介：本研究旨在评估不同硅酸盐基封闭剂（即BioRoot RCS [Septodont，Saint毛尔des福塞，France]、ProRoot ES [Dentsply Sirona，约克，PA]和MTA Fillapex [Angelus，Londrina，PR，Brazil]）对人牙周膜干细胞（PDLSC）细胞因子产生和活力的影响。AH Plus（Dentsply DeTrey GmbH，Konstanz，德国）用作参比物质。研究方法：PDLSC在二维或三维条件下（在0.15%-0.5% PuraMatrix [BD Biosciences，贝德福德，MA]中）培养24小时，洗脱液来自设置的牙髓密封剂。此外，使用体外根模型实验程序评估了根管封闭剂洗脱液的毒性。使用3-（4，5-二甲基-2-噻唑基）-2，5-二苯基-2H-溴化四唑测定法测定PDLSC活力。使用HCYTMAG-60 K-PX 41 Milliplex试剂盒（EMD Millipore，Burlington，MA），将PDLSC培养基用于细胞因子定量（白细胞介素[IL]-6、IL-8、生长调节癌基因、IL-4和IL-10）。结果：在二维培养条件下，BioRoot RCS具有良好的PDLSC存活率。无论稀释度如何，ProRoot ES对PDLSC活力均无影响。即使在最低浸提液稀释度（1：1、1：2和1：4）下，MTA Fillapex也具有强烈的细胞毒性。PuraMatrix中PDLSC的包封倾向于降低密封剂的细胞毒性作用。在三维体外牙根模型实验程序中，BioRoot RCS、ProRoot ES和MTA Fillapex显示了细胞相容性模式。不同的硅酸钙基封闭剂表现出不同的促炎细胞因子产生。BioRoot RCS极大地刺激了PDLSC释放IL-10，并在较小程度上刺激了IL-4（P <0.05）。结论：BioRoot RCS和ProRoot ES不诱导促炎细胞因子，并促进PDLSC的抗炎细胞因子分泌，这可能通过减弱初始炎症反应而产生积极的局部影响。
Introduction: The aim of the present study was to assess the effects of different silicatebased sealers (ie, BioRoot RCS [Septodont, Saint Maur des Fosses, France], ProRoot ES [Dentsply Sirona, York, PA], and MTA Fillapex [Angelus, Londrina, PR, Brazil]) on cytokine production and viability of human periodontal ligament stem cells (PDLSCs). AH Plus (Dentsply DeTrey GmbH, Konstanz, Germany) was used as a reference material. Methods: PDLSCs were cultured either in 2-dimensional or 3-dimensional conditions (in 0.15%-0.5% PuraMatrix [BD Biosciences, Bedford, MA]) for 24 hours with eluates from set endodontic sealers. Additionally, the toxicity of eluates from endodontic sealers was evaluated using an in vitro root model experimental procedure. PDLSC viability was determined using the 3-(4,5-dimethyl-2thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay. PDLSC culture medium was used for cytokine quantification (interleukin [IL]-6, IL-8, growth-regulated oncogene, IL,-4 and IL-10) using the HCYTMAG-60K-PX41 Milliplex kit (EMD Millipore, Burlington, MA). Results: In 2dimensional culture conditions, BioRoot RCS revealed a good PDLSC viability rate. ProRoot ES had no effect on PDLSC viability regardless of the dilution. MTA Fillapex was strongly cytotoxic even at the lowest extract dilutions (1:1, 1:2, and 1:4). Encapsulation of PDLSCs in PuraMatrix tended to decrease the cytotoxic effect of the sealers. In the 3-dimensional in vitro root model experimental procedure, BioRoot RCS, ProRoot ES, and MTA Fillapex revealed a cytocompatibility pattern. Different calcium silicate-based sealers exhibited different proinflammatory cytokine production. BioRoot RCS greatly stimulated the release of IL-10 and, to a lesser degree, IL-4 by PDLSCs (P,.05). Conclusions: BioRoot RCS and ProRoot ES did not induce proinflammatory cytokines and promoted anti-inflammatory cytokine secretion by PDLSCs that may have a positive local impact by attenuating an initial inflammatory response.