Ambient air pollution, H19/DMR methylation in cord blood and newborn size: A pilot study in Zhengzhou City, China
Ambient air pollution, H19/DMR methylation in cord blood and newborn size: A pilot study in Zhengzhou City, China
复制标题
DOI：
10.1016/j.chemosphere.2018.08.140
复制
发表时间：
2018-12-01
期刊：
影响因子：
8.8
通讯作者：
Ba, Yue
中科院分区：
文献类型：
--
作者：
He, Tongkun;Zhu, Jingyuan;Ba, Yue
产前接触空气污染物被认为与不良的出生结果有关。然而，潜在的机制，特别是表观遗传修饰效应，仍不清楚。本研究旨在探讨空气污染、H19/DMR甲基化水平与出生体重和身高之间的关系。从郑州市后寨中心医院共招募了527对母婴。收集了研究期间的空气污染数据。采用实时荧光定量PCR检测母血和脐带血H19启动子区甲基化和H19 DMR。采用Ridge回归分析妊娠期空气污染物暴露与H19/DMR甲基化、出生体重和出生身高的关系。结果表明，产前暴露于NO2与脐带血中较高的H19甲基化有关。而SO2和PM10暴露分别与较低的H19和H19 DMR甲基化有关。在妊娠期分层后，还发现脐带血中H19甲基化与PK10暴露有关。此外，产前接触空气污染物也与出生体重和身高有关。具体而言，随着母亲在整个妊娠期间二氧化硫暴露量的增加，出生体重和体长均显著降低。而出生体重和出生长度则随二氧化氮暴露而显著增加。分层分析还发现，在不同的孕期，PM10暴露与出生尺寸之间存在关联。综上所述，脐带血基因甲基化水平可能与产前环境暴露有关。出生体重和身高与产前环境暴露和遗传因素有关。(C) 2018 Elsevier Ltd.版权所有。
Prenatal exposure to air pollutants is believed to be associated with adverse birth outcomes. However, the potential mechanisms, especially the epigenetic modified effects, still remain unclear. This study was designed to explore the association of air pollution, H19/DMR methylation levels, and birth weight and length. A total of 527 mother-infant pairs were recruited from Houzhai Center Hospital, Zhengzhou. Air pollution data during the study period was collected. The methylation at H19 promoter region and H19 DMR in maternal and cord bloods were determined using real-time PCR analysis. Ridge regression was used to analyze the association of air pollutants exposure during gestation with H19/DMR methylation and birth weight and length respectively. Results showed that prenatal exposure to NO2 was associated with higher H19 methylation in cord blood. Whereas SO2 and PM10 exposure were associated with lower H19 and H19 DMR methylation respectively. After stratification by pregnancy trimesters, the association of H19 methylation in cord blood with PK10 exposure also was found. Furthermore, prenatal exposures to air pollutants also were associated with birth weight and length. Specifically, with the increase of maternal SO2 exposure during the entire pregnancy, birth weight and length significantly decreased. While birth weight and birth length were significantly increased with NO2 exposure. The stratified analysis also found the associations between PM10 exposure and birth sizes in different trimesters. In conclusion, the gene methylation level in cord blood might be associated with prenatal environmental exposures. Birth weight and length were associated with both prenatal environmental exposures and genetic factors. (C) 2018 Elsevier Ltd. All rights reserved.