Preparation and characterization of composite nanofibers of polycaprolactone and nanohydroxyapatite for osteogenic differentiation of mesenchymal stem cells
Preparation and characterization of composite nanofibers of polycaprolactone and nanohydroxyapatite for osteogenic differentiation of mesenchymal stem cells
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DOI：
10.1016/j.colsurfb.2011.03.038
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发表时间：
2011-08-01
影响因子：
5.8
通讯作者：
Chang, Yin-Shin
中科院分区：
文献类型：
--
作者：
Chen, Jyh-Ping;Chang, Yin-Shin
采用静电纺丝法制备了纳米羟基磷灰石（nHAP）/聚己内酯（PCL）纳米复合材料，得到PCL/nHAP纳米纤维。制造具有相似直径（340 +/-30nm）但不同nHAP浓度（0-50%）的纳米纤维，并研究其用于骨髓间充质干细胞（MSC）的生长和成骨分化。通过扫描电子显微镜（SEM）、热重分析、X射线衍射、傅里叶变换红外光谱和机械拉伸测试对其物理化学性质进行详细分析。嵌入纳米纤维中的nHAP颗粒（类似于30 nm直径）增加了纳米纤维膜的极限应力和弹性模量，同时降低了失效时的应变。当在成骨刺激条件下在纳米纤维上培养时，MSC显示正常的表型细胞形态，以及从SEM观察和碱性磷酸酶活性测定的时间依赖性矿化和成骨分化。纳米纤维可以支持间充质干细胞的生长，而不影响其成骨分化能力长达21天，并且nHAP对细胞分化的增强与其在纳米纤维中的浓度正相关。能量色散X-射线分析的Ca和P元素表明在细胞表面的矿物质沉积。结果表明，含50%nHAP的纳米纤维的矿化程度显著提高，其Ca/P比值与骨组织相近，表明电纺复合PCL/nHAP纳米纤维膜适合于骨组织工程用MSCs的矿化。(C)2011 Elsevier B. V.保留所有权利。
Nanocomposites of nanohydroxyapatite (nHAP) dispersed in poly(epsilon-caprolactone) (PCL) were prepared by electrospinning (ES) to obtain PCL/nHAP nanofibers. Nanofibers with similar diameters (340 +/- 30 nm) but different nHAP concentrations (0-50%) were fabricated and studied for growth and osteogenic differentiation of bone marrow mesenchymal stem cells (MSCs). The nanofibrous membranes were subjected to detailed analysis for its physicochemical properties by scanning electron microscopy (SEM), thermogravimetric analysis, X-ray diffraction, Fourier-transform infrared spectroscopy, and mechanical tensile testing. nHAP particles (similar to 30 nm diameter) embedded in nanofibers increased the nanofibrous membrane's ultimate stress and the elastic modulus, while decreased the strain at failure. When cultured under an osteogenic stimulation condition on nanofibers, MSCs showed normal phenotypic cell morphology, and time-dependent mineralization and osteogenic differentiation from SEM observations and alkaline phosphatase activity assays. The nanofibers could support the growth of mesenchymal stern cells without compromising their osteogenic differentiation capability up to 21 days and the enhancement of cell differentiation by nHAP is positively correlated with its concentration in the nanofibers. Energy dispersive X-ray analysis of Ca and P elements indicated mineral deposits on the cell surface. The mineralization extent was significantly raised in nanofibers with 50% nHAP where a Ca/P ratio similar to that of bone was found. The present study indicated that electrospun composite PCL/nHAP nanofibrous membranes are suitable for mineralization of MSCs intended for bone tissue engineering. (C) 2011 Elsevier B.V. All rights reserved.