Plasma protein interactions with Pluronic(TM)-treated colloids
Plasma protein interactions with Pluronic(TM)-treated colloids
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DOI：
10.1016/0927-7765(96)01269-6
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发表时间：
1996-07-31
影响因子：
5.8
通讯作者：
Caldwell, KD
中科院分区：
文献类型：
--
作者：
Li, JT;Caldwell, KD
众所周知，疏水性聚苯乙烯胶体可以迅速从溶液中吸收大量蛋白质，包括关键的血浆蛋白白蛋白和纤维蛋白原。然而，当包覆了含有聚氧乙烯(PEO)的嵌段共聚物时，这些颗粒变得不太容易吸附蛋白质。蛋白质的排斥性被认为受到三个重要因素的影响：吸附聚合物的表面浓度、吸附层的厚度和聚合物链的表面迁移率。在给定的表面浓度下，从选定的共聚物吸附络合物与人纤维蛋白原的相互作用可以看出，厚度的增加和动力学的增加与蛋白质吸附的减少直接相关。Pluronic(TM)F108在所研究的嵌段共聚物中具有最高的相对分子质量和最长的PEO链，它显著地抑制了纤维蛋白原在聚苯乙烯(PS)胶体上的吸附，促使人们详细研究了这种经表面活性剂处理的颗粒表面与其他蛋白质的相互作用。将包覆的颗粒暴露在全血浆中会产生一定的聚合物置换，伴随而来的是摄取有限数量的蛋白质。这种吸收不会导致聚集，也不会增加每个颗粒的大小。相反，正如一项配套研究显示的那样，稳定的F108涂层所建立的排斥层似乎与这种涂层PS胶体的血液循环延长有关。
Hydrophobic polystyrene colloids are known to rapidly take up numerous proteins from solution, including the key plasma proteins albumin and fibrinogen. However, when coated with poly(ethylene oxide) (PEO)-containing block copolymers these particles become much less prone to protein adsorption. The noted protein repulsion is thought to be influenced by three important factors: the surface concentration of the adsorbed polymers, the thickness of the adsorbed layer and the surface mobility of the polymer chains. At a given surface concentration, the increase in both thickness and dynamics is directly correlated with a decrease in protein adsorption, as seen from the interactions of selected copolymer adsorption complexes with human fibrinogen. The significant suppression of fibrinogen adsorption to polystyrene (PS) colloids coated with Pluronic(TM) F108, which has the highest molecular weight and longest PEO chains among the studied block copolymers, prompted a detailed examination of the interaction of such surfactant-treated particulate surfaces with other proteins. Exposure of the coated particles to whole plasma gives rise to a certain polymer displacement with concomitant uptake of a limited amount of protein. This uptake does not result in aggregation nor does it add to the size of each particle. Rather, as shown in a companion study, the repulsion layer established by the stable F108 coating appears to be correlated with the prolonged blood circulation of such coated PS colloids.