Cloned murine T200 (Ly-5) cDNA reveals multiple transcripts within B- and T-lymphocyte lineages.
Cloned murine T200 (Ly-5) cDNA reveals multiple transcripts within B- and T-lymphocyte lineages.
复制标题
克隆的小鼠 T200 (Ly-5) cDNA 揭示了 B 和 T 淋巴细胞谱系中的多个转录物。
DOI：
10.1073/pnas.84.1.161
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发表时间：
1987
影响因子：
11.1
通讯作者：
Raschke,WC
中科院分区：
文献类型：
--
作者：
Raschke,WC
细胞表面糖蛋白的鼠T200家族以发育调节的方式在造血谱系细胞类型上表达，不同的淋巴细胞亚群表达交叉反应但结构不同的形式。为了研究这些形式之间的差异和这些差异的调节，小鼠T200 cDNA克隆分离通过使用通过消减杂交获得的探针。该程序利用T200+ L-细胞转染子和亲本Ltk-细胞系。对1.9kb的cDNA克隆进行测序，发现其含有羧基端331个氨基酸的编码区和0.9kb的3'非翻译区。当序列与大鼠序列重叠时，观察到80-90%的同源性。RNA印迹分析显示，B系细胞系表达与合成的T200糖蛋白的大小差异相关的5.6-kb或6.5-kb mRNA。类似地，在T细胞谱系中，辅助T细胞克隆和细胞毒性T细胞克隆分别表达5.6kb和5.9kb的T200转录物，这与它们的T200糖蛋白的不同大小相关。发现T细胞系的T200阴性突变体表达全长T200 mRNA，尽管水平降低。
The murine T200 family of cell-surface glycoproteins is expressed on hematopoietic lineage cell types in a developmentally regulated manner with different lymphocyte subpopulations expressing cross-reactive but structurally distinct forms. To investigate the differences between these forms and the regulation of these differences, murine T200 cDNA clones were isolated by using a probe obtained by subtractive hybridization. This procedure made use of a T200+ L-cell transfectant and the parent Ltk- cell line. The 1.9-kilobase (kb) cDNA clone was sequenced and found to contain the coding region of the COOH-terminal 331 amino acids and 0.9 kb of 3' untranslated region. Where the sequence overlapped with the rat sequence, 80-90% homology was observed. RNA blot analysis revealed that B-lineage cell lines express either a 5.6-kb or a 6.5-kb mRNA correlating to the size difference of the T200 glycoprotein synthesized. Similarly, in the T-cell lineage a helper T-cell clone and a cytotoxic T-cell clone express T200 transcripts of 5.6 kb and 5.9 kb, respectively, which correlate with the distinct sizes of their T200 glycoproteins. A T200-negative mutant of a T-cell line was found to express full-length T200 mRNA, although at a diminished level.