Towards a quantitative SERS approach - online monitoring of analytes in a microfluidic system with isotope-edited internal standards
Towards a quantitative SERS approach - online monitoring of analytes in a microfluidic system with isotope-edited internal standards
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DOI：
10.1002/jbio.200810069
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发表时间：
2009-04-01
影响因子：
2.8
通讯作者：
Popp, Juergen
中科院分区：
文献类型：
--
作者：
Maerz, Anne;Ackermann, Katrin R.;Popp, Juergen
在这篇文章中，提出了一种使用表面增强拉曼光谱（SERS）进行定量测量的新方法。将同位素编辑内标的应用与基于液-液分段流的流通式 SERS 检测方法的优点相结合，似乎是定量 SERS 分析的一种有前景的方法。对于此处讨论的研究，采用了新设计的流动池，并根据灰度值测量测试了理想的混合效率。使用杂芳族尼古丁和吡啶各自的氘代同位素异构体作为内标进行测量表明，将同位素标记的内标整合到所用的液-液两相分段流中可产生可重复且可比较的 SERS 光谱，而与所用的胶体无关。通过在微流体装置中实施内标，可以补偿胶体特性对 SERS 活性的影响。因此，解决了所需纳米颗粒溶液的批次间再现性差的问题。据我们所知，这些是结合上述概念的首次测量，以纠正各个胶体增强行为的差异。 (C) 2009 年，WILEY-VCH Verlag GmbH & Co. KGaA，魏因海姆。
In this contribution a new approach for quantitative measurements using surface-enhanced Raman spectroscopy (SERS) is presented. Combining the application of isotope-edited internal standard with the advantages of the liquid-liquid segmented-flow-based approach for flow-through SERS detection seems to be a promising means for quantitative SERS analysis. For the investigations discussed here a newly designed flow cell, tested for ideal mixing efficiency on the basis of grayscale-value measurements, is implemented. Measurements with the heteroaromatics nicotine and pyridine using their respective deuterated isotopomers as internal standards show that the integration of an isotopically labeled internal standard in the used liquid-liquid two-phase segmented flow leads to reproducible and comparable SERS spectra independent from the used colloid. With the implementation of an internal standard into the microfluidic device the influence of the properties of the colloid on the SERS activity can be compensated. Thus, the problem of a poor batch-to-batch reproducibility of the needed nanoparticle solutions is solved. To the best of our knowledge these are the first measurements combining the above mentioned concepts in order to correct for differences in the enhancement behaviour of the respective colloid. (C) 2009 by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.