Dominant TNF-α+ Mycobacterium tuberculosis-specific CD4+ T cell responses discriminate between latent infection and active disease.
Dominant TNF-α+ Mycobacterium tuberculosis-specific CD4+ T cell responses discriminate between latent infection and active disease.
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DOI：
10.1038/nm.2299
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发表时间：
2011-03
期刊：
影响因子：
82.9
通讯作者：
Pantaleo G
中科院分区：
文献类型：
--
作者：
Harari A;Rozot V;Bellutti Enders F;Perreau M;Stalder JM;Nicod LP;Cavassini M;Calandra T;Blanchet CL;Jaton K;Faouzi M;Day CL;Hanekom WA;Bart PA;Pantaleo G
活动性结核分枝杆菌（Mtb）感染的快速诊断仍然是临床和实验室面临的挑战。我们通过多色流式细胞术分析了Mtb特异性T细胞的细胞因子谱（干扰素 -γ（IFN -γ）、肿瘤坏死因子 -α（TNF -α）和白细胞介素 -2（IL -2））。我们研究了潜伏性Mtb感染和活动性结核病患者中Mtb特异性CD4 + T细胞反应。结果显示，在活动性疾病患者中，单阳性TNF -α Mtb特异性CD4 + T细胞的比例大幅增加，并且该参数是诊断活动性疾病与潜伏感染的最强预测指标。我们在一组101名研究者未知其结核病诊断的受试者中验证了该参数的使用。基于流式细胞术的检测方法的敏感性和特异性分别为67%和92%，阳性预测值为80%，阴性预测值为92.4%。因此，单阳性TNF -α Mtb特异性CD4 + T细胞的比例是快速诊断活动性结核病的一种新工具。
Rapid diagnosis of active Mycobacterium tuberculosis (Mtb) infection remains a clinical and laboratory challenge. We have analyzed the cytokine profile (interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α) and interleukin-2 (IL-2)) of Mtb-specific T cells by polychromatic flow cytometry. We studied Mtb-specific CD4+ T cell responses in subjects with latent Mtb infection and active tuberculosis disease. The results showed substantial increase in the proportion of single-positive TNF-α Mtb-specific CD4+ T cells in subjects with active disease, and this parameter was the strongest predictor of diagnosis of active disease versus latent infection. We validated the use of this parameter in a cohort of 101 subjects with tuberculosis diagnosis unknown to the investigator. The sensitivity and specificity of the flow cytometry–based assay were 67% and 92%, respectively, the positive predictive value was 80% and the negative predictive value was 92.4%. Therefore, the proportion of single-positive TNF-α Mtb-specific CD4+ T cells is a new tool for the rapid diagnosis of active tuberculosis disease.
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影响因子：
15.3
作者：
Harari, Alexandre;Bart, Pierre-Alexandre;Stoehr, Wolfgang;Tapia, Gonzalo;Garcia, Miguel;Medjitna-Rais, Emmanuelle;Burnet, Severine;Cellerai, Cristina;Erlwein, Otto;Barber, Tristan;Moog, Christiane;Liljestrom, Peter;Wagner, Ralf;Wolf, Hans;Kraehenbuhl, Jean-Pierre;Esteban, Mariano;Heeney, Jonathan;Frachette, Marie-Joelle;Tartaglia, James;McCormack, Sheena;Babiker, Abdel;Weber, Jonathan;Pantaleo, Giuseppe
通讯作者：
Pantaleo, Giuseppe
影响因子：
64.8
作者：
通讯作者：
--
影响因子：
4.9
作者：
METZ, CE
通讯作者：
METZ, CE
影响因子：
168.9
作者：
Ewer, K;Deeks, J;Lalvani, A
通讯作者：
Lalvani, A
影响因子：
20.3
作者：
Betts, Michael R.;Nason, Martha C.;Koup, Richard A.
通讯作者：
Koup, Richard A.