Functional properties of rat brain sodium channels expressed in a somatic cell line.
Functional properties of rat brain sodium channels expressed in a somatic cell line.
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体细胞系中表达的大鼠脑钠通道的功能特性。
DOI：
10.1126/science.2154850
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Catterall,WA
中科院分区：
文献类型：
--
作者：
Scheuer,T;Auld,VJ;Boyd,S;Offord,J;Dunn,R;Catterall,WA
用编码大鼠脑RIIAsodium通道α亚基的互补DNA转染中国仓鼠卵巢细胞，可表达具有快速、电压依赖性激活和失活特性的功能性钠通道。这些通道介导的钠电流被河豚毒素抑制，被缬草碱持续激活，被α-蝎毒延长，表明神经毒素受体位点1至3以功能形式存在。仅RIIAsodium通道α亚基cDNA就足以在哺乳动物体细胞中稳定表达具有预期动力学和药理学特性的功能性钠通道。
Transfection of Chinese hamster ovary cells with complementary DNA encoding the RIIAsodium channel α subunit from rat brain led to expression of functional sodium channels with the rapid, voltage-dependent activation and inactivation characteristic of sodium channels in brain neurons. The sodium currents mediated by these transfected channels were inhibited by tetrodotoxin, persistently activated by veratridine, and prolonged byLeiurusα-scorpion toxin, indicating that neurotoxin receptor sites 1 through 3 were present in functional form. The RIIAsodium channel α subunit cDNA alone is sufficient for stable expression of functional sodium channels with the expected kinetic and pharmacological properties in mammalian somatic cells.