Impact of phytoplankton size and physiology on particulate optical properties determined with scanning flow cytometry
Impact of phytoplankton size and physiology on particulate optical properties determined with scanning flow cytometry
复制标题
浮游植物大小和生理学对扫描流式细胞术测定的颗粒光学特性的影响
DOI：
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发表时间：
2015
期刊：
影响因子：
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通讯作者：
P. Donaghay
中科院分区：
文献类型：
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作者：
M. McFarland;J. Rines;J. Sullivan;P. Donaghay
浮游植物的层和斑块在垂直方向上的亚米尺度和在水平方向上的公里尺度是沿海海洋的共同特征。这些细胞的异质分布是其种群动态和远洋生态系统功能的基础。为了更好地了解这些小尺度上的生物过程，开发了基于高分辨率原位光学测量来评估浮游植物群落组成和生理特征的方法。离散样品的扫描流式细胞术被用来确定浮游植物和非藻类颗粒丰度，大小和色素含量的光谱形状和相对大小的颗粒衰减，吸收，散射和后向散射系数的影响。颗粒衰减的斜率随浮游植物的大小和形态而变化，颗粒吸收的斜率和散射吸收比主要随细胞色素含量而变化，后向散射比主要随非藻类颗粒的相对丰度而变化。从光学测量在小的空间和时间尺度上的颗粒和浮游植物的特性的测定进行了测试与2个独立的高分辨率数据集收集的现场自主剖析系统。这些高分辨率的光学数据与流式细胞仪样品分析的比较，一般同意与先前确定的关系，但表明，复杂的形态学的大型殖民硅藻可能会导致高于预期的颗粒衰减斜率。高分辨率的数据显示，在社会规模结构和生理学的变化，将难以可视化与离散的样品或措施的总叶绿素浓度。
Layers and patches of phytoplankton at sub-meter scales in the vertical dimension and kilometer scales in horizontal dimensions are common features in the coastal ocean. These heterogeneous distributions of cells are fundamental to their population dynamics and the function of pelagic ecosystems. To better understand biological processes at these small scales, methods were developed to assess phytoplankton community composition and physiological characteristics based on high-resolution, in situ optical measurements. Scanning flow cytometry of discrete samples was used to determine the effects of phytoplankton and non-algal particle abundance, size, and pigment content on the spectral shape and relative magnitude of particulate attenuation, absorption, scatter, and backscatter coefficients. The slope of particulate attenuation varied with phytoplankton size and morphology, the slope of particulate absorption and the ratio of scatter to absorption varied primarily with cellular pigment content, and the backscatter ratio varied primarily with the relative abundance of non-algal particles. Determination of particle and phytoplankton characteristics from optical measurements over small spatial and temporal scales was tested with 2 independent high-resolution data sets collected from an in situ autonomous profiling system. Comparison of these high-resolution optical data with flow cytometric sample analyses generally agreed with the previously determined relationships but suggest that complex morpho logy of large colonial diatoms may result in higher than expected particulate attenuation slopes. High-resolution data revealed variations in community size structure and physiology that would be difficult to visualize with discrete samples or measures of total chlorophyll concentration.