Proteomic analysis of differences in barley (Hardeum vulgare) malts with distinct filterability by DIGE
Proteomic analysis of differences in barley (Hardeum vulgare) malts with distinct filterability by DIGE
复制标题
通过 DIGE 对具有不同过滤性的大麦 (Hardeum vulgare) 麦芽差异进行蛋白质组学分析
DOI：
10.1016/j.jprot.2013.05.038
复制
发表时间：
2013-11-20
影响因子：
3.3
通讯作者：
Lu, Jian
中科院分区：
文献类型：
--
作者：
Jin, Zhao;Li, Xiao-Min;Lu, Jian
过滤性能是大麦麦芽的重要质量参数，对啤酒的生产效率和质量有重要影响。研究发现，大麦代谢能力的差异，而不是大麦大分子初始含量的差异，是造成中国麦芽过滤性能差异的主要原因。采用基于荧光差异凝胶电泳法(DGE)的比较蛋白质组学方法对两个品种的4种商品麦芽的蛋白质进行了定量分析，利用MALDI-TOF/TOF质谱仪从40种代谢蛋白质中鉴定出51个品种差异点，主要包括水解酶和病原菌相关蛋白质。根据它们的功能分析和品种间的丰度比较，初步提出了可过滤的有利蛋白和不利蛋白。进一步研究了两种最显著的差异蛋白质--β-淀粉酶和丝氨酸Z7，以验证它们对丹耳麦芽过滤性能的影响。这些结果为大麦育种者和麦芽师提供了改善麦芽过滤性能的生物标记。生物学意义据我们所知，这是首次对与大麦麦芽过滤性能相关的代谢蛋白进行全面研究，为改善麦芽过滤性能提供了线索。利用2D-DGE对丹麦芽和麦特卡夫麦芽中代谢蛋白表达水平的明显差异进行了研究，这对于品种比较、解释大麦麦芽的过滤性能和其他品质的关键蛋白质来说是一个有价值的工具。通过对麦芽过滤性能等方面生物标志物的探索，将提高啤酒酿造的效率和质量，减少外源水解酶的使用，扩大我国啤酒大麦的市场。本文是题为：翻译植物蛋白质组学特刊的一部分。(C)2013爱思唯尔B.V.保留所有权利。
Filterability is an essential quality parameter of barley malt and significantly impacts productive efficiency and quality of beer. In the study, differences of metabolic capability, rather than of initial contents of macromolecules in barleys, were found to be the main reason for malt filterability gap between the widely used cultivars Dan'er and Metcalfe in China. Comparative proteomics based on fluorescent difference gel electrophoresis (DIGE) was employed to quantitatively analyze proteins of four commercial malts belonging to the two cultivars, and 51 cultivar-differential spots were identified to 40 metabolic proteins by MALDI-TOF/TOF mass spectrometry, mainly including hydrolases and pathogen-related proteins. According to their function analysis and abundance comparison between cultivars, filterability-beneficial and -adverse proteins were putatively proposed. Two most remarkable differential proteins, beta-amylase and serpin Z7, were further investigated to verify their effects on Dan'er malt filterability. These results provide biological markers for barley breeders and maltsters to improve malt filterability.Biological significanceTo the best of our knowledge, this is the first report of comprehensive investigation of metabolic proteins related to wort filterability of barley malts, and sheds light on clues for filterability improvement. Visible differences in the expression level of metabolic proteins between Dan'er and Metcalfe malts using 2D-DIGE signify a valuable tool for cultivar comparison, illustration of key proteins responsible for filterability and even other qualities of barley malts. And with these explorations on biomarkers of malt filterability and other aspects, there will be higher efficiency and quality of beer brewing, less application of exogenous hydrolases and more expending market for Chinese malting barleys. This article is part of a Special Issue entitled: Translational Plant Proteomics. (C) 2013 Elsevier B.V. All rights reserved.