Immunohistochemical Characterization of Hemangiopericytomas and Other Spindle Cell Tumors in the Dog
Immunohistochemical Characterization of Hemangiopericytomas and Other Spindle Cell Tumors in the Dog
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狗血管外皮细胞瘤和其他梭形细胞肿瘤的免疫组织化学特征
DOI：
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发表时间：
1996
期刊：
影响因子：
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通讯作者：
J. M. Mulas
中科院分区：
文献类型：
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作者：
J. Pérez;M. J. Bautista;E. Rollón;F. D. Lara;L. Carrasco;J. M. Mulas
应用单克隆抗体（HHF 35）和福尔马林固定石蜡包埋标本，对45例犬血管外皮细胞瘤（CHP）中肌肉肌动蛋白的表达进行了免疫组化研究。波形蛋白，结蛋白，细胞角蛋白，溶菌酶，因子VIII相关抗原，S-100蛋白，和胶质细胞酸性蛋白的分布进行了研究，在CHP和一些犬软组织肿瘤（7个纤维肉瘤，7个良性神经鞘瘤，7个良性纤维组织细胞瘤，6个平滑肌瘤）作为对照鉴别诊断。所有CHP和对照肿瘤表达波形蛋白。23例CHP表达肌肉肌动蛋白，而所有分析的对照肿瘤均为肌肉肌动蛋白阴性，平滑肌瘤除外。在肌肌动蛋白和波形蛋白阳性的CHP中，1例结蛋白阳性，2例溶菌酶阳性，9例S-100蛋白阳性，可重新分类为平滑肌肉瘤。在肌肉肌动蛋白阴性和波形蛋白阳性的CHP中，7个表达S-100蛋白。此外，S-100蛋白在5例神经鞘瘤中检出。所有分析的CHP和对照肿瘤的细胞角蛋白、因子VIII相关抗原和胶质细胞酸性蛋白均为阴性。我们的研究结果支持假设的周细胞起源的CHP，并建议，肌肉肌动蛋白，结蛋白，波形蛋白和溶菌酶可能是有用的犬梭形细胞肿瘤的鉴别诊断，但不是所有这些肿瘤都可以确定与这些肿瘤组织标志物。
The immunohistochemical expression of muscle actin has been studied in 45 canine hemangiopericytomas (CHP) using a monoclonal antibody (HHF35) and formalin-fixed, paraffin-embedded specimens. The distribution of vimentin, desmin, cytokeratins, lysozyme, factor VIII-related antigen, S-100 protein, and glial fibrillary acidic protein was studied both in CHP and in some canine soft-tissue neoplasms (seven fibrosarcomas, seven benign schwannomas, seven benign fibrous histiocytomas, and six leiomyosarcomas) used as controls for differential diagnosis. All CHP and control tumors expressed vimentin. Twenty-three CHP expressed muscle actin, whereas all control tumors analyzed were muscle actin-negative, with the exception of leiomyosarcomas. Among muscle actin- and vimentin-positive CHP, one case could be reclassified as leiomyosarcoma because it was desmin-positive, two cases expressed lysozyme, and nine cases expressed S-100 protein. Among muscle actin-negative and vimentin-positive CHP, seven expressed S-100 protein. In addition, S-100 protein was detected in five schwannomas. All CHP and control tumors analyzed were negative for cytokeratins, factor VIII-related antigen, and glial fibrillary acidic protein. Our results support the hypothesis of a pericytic origin of CHP, and suggest that muscle actin, desmin, vimentin, and lysozyme could be useful for the differential diagnosis of canine spindle cell tumors, but not all these neoplasms can be identified with these tumor tissue markers.