Comparison of different DNA preparation protocols for PCR diagnosis of Human African Trypanosomosis in Cote d'Ivoire
Comparison of different DNA preparation protocols for PCR diagnosis of Human African Trypanosomosis in Cote d'Ivoire
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DOI：
10.1016/s0001-706x(02)00029-3
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发表时间：
2002-06-01
期刊：
影响因子：
2.7
通讯作者：
Garcia, A
中科院分区：
文献类型：
--
作者：
Solano, P;Jamonneau, V;Garcia, A
在对象牙海岸博农的昏睡病疫源地进行医学调查期间，用布鲁氏锥虫特异性引物(来自寄生虫学99(1989)57的1-2)对从血液样本中提取的DNA进行了聚合酶链式反应。用螯合树脂对全血或以两种不同方法制备的棕黄色涂层进行DNA纯化。基于全血的制剂比使用黄褐色涂层的制剂效果更好。第一种方法对寄生虫学确诊患者的敏感性为100%，特异性为92%。然而，有人指出了该技术的重复性问题，特别是在来自血清学阳性但明显是寄生虫感染者的样本上。提示该方法可用于非洲人锥虫病的诊断，但对其他引物的使用还需进一步研究。(C)2002 Elsevier Science B.V.保留所有权利。
During a medical survey the sleeping sickness focus in Bonon, Ivory Coast, PCR with Trypanosoma brucei specific primers (TBR 1-2 from Parasitology 99 (1989) 57) was tested on DNA derived from blood samples. DNA purification using a chelating resin was performed either on whole blood or on the buffy coat prepared in two different ways. The preparation based on whole blood performed better than those using the buffy-coat. Using this first method, the sensitivity was 100% on parasitologically confirmed patients, and the specificity was 92%. However, problems of reproducibility of the technique were pointed out, particularly on samples from serologically positive but apparently aparasitemic individuals. It is suggested that the PCR could help in the diagnosis of Human African Trypanosomosis, but the use of other primers should be investigated. (C) 2002 Elsevier Science B.V. All rights reserved.