Integration of hydrodynamic and odorant inputs by local interneurons of the crayfish deutocerebrum
Integration of hydrodynamic and odorant inputs by local interneurons of the crayfish deutocerebrum
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DOI：
10.1242/jeb.01827
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发表时间：
2005-10-01
影响因子：
2.8
通讯作者：
Mellon, DF
中科院分区：
文献类型：
--
作者：
Mellon, DF
用细胞内电极记录克氏原螯虾中脑嗅叶局部中间神经元的活动。细胞类似于以前研究的中央目标的嗅觉受体神经元的侧触角鞭毛进行了专门检查，其响应水动力刺激。启动水运动过去的触角鞭毛，局限在嗅觉计，诱发三相兴奋性抑制兴奋性突触后电位持续2秒，产生尖峰的反应序列的去极化阶段。气味脉冲无缝嵌入水脉冲过去的触角引起纯粹的兴奋性，剂量依赖性突触后反应和相关的尖峰列车。对水的反应的初始阶段的潜伏期约为对气味的反应的潜伏期的一半，这表明不同的传入通路分别参与对流体动力学和气味刺激的反应。在一些嗅叶中间神经元，类似于先前描述的细胞分类为I型，联合刺激的流体发作和气味诱发的反应，是两次的振幅总和响应单独的流体动力学或气味刺激，这表明嗅觉反应增强流体动力学输入。个人的至少一个其他类的一阶interneuron响应流体动力学和气味刺激偶尔记录。这些结果表明，化学和机械信息的多模态整合发生在一阶感觉中间神经元的水平：在小龙虾的大脑。
Intracellular electrodes were used to record from local interneurons in the olfactory lobes of the midbrain in the crayfish Procambarus clarkii. Cells that resembled previously studied central targets of olfactory receptor neurons on the lateral antennular flagellum were specifically examined for their responses to hydrodynamic stimuli. Initiation of water movement past the antennular flagellum, confined within an olfactometer, evoked a triphasic excitatory-inhibitory-excitatory postsynaptic potential lasting up to 2 s that generated spikes on depolarizing phases of the response sequence. Odorant pulses seamlessly imbedded in the water pulse past the antennule evoked purely excitatory, dose-dependent postsynaptic responses and associated spike trains. The latency of the initial phase of the response to water was approximately half as long as the latency of the response to odorant, suggesting that different afferent pathways are involved in responses to hydrodynamic and odorant stimuli, respectively. In some olfactory lobe interneurons that resembled previously described cells classified as Type I, conjoint stimulation of fluid onset and odorant evoked responses that were twice the amplitude of the summed response to either hydrodynamic or odorant stimulation alone, suggesting that the olfactory responses were potentiated by hydrodynamic input. Individuals of at least one other class of first-order interneuron that responded to both hydrodynamic and odorant stimulation were occasionally recorded from. These results indicate that multimodal integration of chemical and mechanical information occurs at the level of first-order sensory interneurons: in the crayfish brain.