p53 expression, DNA content and cell proliferation in primary and synchronous metastatic lesions from ovarian surface epithelial-stromal tumours
p53 expression, DNA content and cell proliferation in primary and synchronous metastatic lesions from ovarian surface epithelial-stromal tumours
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DOI：
10.1016/0959-8049(96)00101-3
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发表时间：
1996-07-01
影响因子：
8.4
通讯作者：
Silvestrini, R
中科院分区：
文献类型：
--
作者：
Daidone, MG;Benini, E;Silvestrini, R
本研究的目的是探讨原发性和转移性卵巢癌病灶之间的生物异质性，作为阐明临床进展步骤的一种手段。对61例未经治疗的卵巢表面上皮间质肿瘤患者的癌组织进行了检查。在原发性肿瘤和相应的同步转移瘤中，通过PAb 1801抗体免疫细胞化学检测p53表达，通过流式细胞术评价DNA含量，并通过[H-3]胸苷标记指数评价细胞增殖。p53阳性的频率在原发性（62%）和转移性（66%）部位相似，97%的病例中同一患者的两个病灶之间的一致性。同样，同一患者的原发性和转移性病变中非整倍体频率（80%）和DNA指数可重叠，一致性为94%。非整倍体的频率在p53阳性的病变高于p53阴性。观察到原发性和转移性病变的增殖活性总体一致性较差（r（s）= 0.44），这是由于网膜相对于原发性肿瘤的异质性，这主要在p53阳性癌症中明显。相反，腹膜、腹部和盆腔病变的细胞增殖在88%的患者中与原发性肿瘤的细胞增殖定性相似。版权所有（C）1996 Elsevier Science Ltd
The aim of this study was to investigate biological heterogeneity between primary and metastatic ovarian cancer lesions from individual patients as a means of elucidating steps in clinical progression. Cancer tissue from 61 untreated patients with ovarian surface epithelial-stromal tumours was examined. p53 expression detected immunocytochemically by the PAb1801 antibody, DNA content evaluated by flow cytometry, and cell proliferation evaluated as the [H-3]thymidine labelling index were investigated in primary tumours and corresponding synchronous metastases. The frequency of p53 positivity was similar in primary (62%) and metastatic (66%) sites, with an agreement between the two lesions from the same patient in 97% of the cases. Similarly, aneuploidy frequency (80%) and DNA indices were superimposable in primary and metastatic lesions from the same patient, with a 94% agreement. The frequency of aneuploidy was higher in p53-positive than in p53-negative lesions. An overall poor agreement (r(s) = 0.44) was observed for proliferative activity of primary and metastatic lesions, due to a heterogeneous profile in omental with respect to primary tumours, which was mainly evident in p53-positive cancers. Conversely, cell proliferation of peritoneal, abdominal and pelvic lesions was qualitatively similar to that of the primary tumour in 88% of patients. Copyright (C) 1996 Elsevier Science Ltd